喹诺酮药物与DNA分子相互作用的紫外光谱研究

J4 ›› 2011, Vol. 46 ›› Issue (7): 11-16.

喹诺酮药物与DNA分子相互作用的紫外光谱研究

张碧程¹,胡林²*,刘艳辉²,张婧¹

1. 贵州大学生命科学院, 贵阳贵州 550025; 2. 贵州大学理学院软凝聚态物理实验室, 贵阳贵州 550025

收稿日期:2010-12-14 出版日期:2011-07-20 发布日期:2011-09-08
通讯作者: 胡林(1953- ),女,教授,博导,研究领域为软凝聚态物理.Email:hulin53@sina.com
作者简介:张碧程(1985- ),男,硕士研究生,研究方向为分子生物物理学.Email:zhangxiaopengcheng@163.com
基金资助:
国家自然科学基金项目(11047022);贵州省社科公关项目(黔科合0Z字［2009］2);贵州省省长基金资助项目(黔省专合字［2010］16号);贵州省科技基金项目(黔科合J字［2010］2141号);贵州大学引进人才基金项目(贵大人基合字［2009］001号)

Study on interaction of both quinolones with DNA by ultraviolet spectroscopy

ZHANG Bi-cheng¹, HU Lin²*, LIU Yan-hui², ZHANG Jing¹

1. College of Life Sciences, Guizhou University, Guiyang 550025, Guizhou, China;
2. College of Sciences, Guizhou University, Guiyang 550025, Guizhou, China

Received:2010-12-14 Online:2011-07-20 Published:2011-09-08

摘要/Abstract

摘要：

在不同时间、温度、浓度等条件下,利用紫外分光光度法研究氟罗沙星(FLRX)、洛美沙星(LMF)与鲑鱼精DNA的相互作用。结果表明:鲑鱼精DNA紫外吸收值分别随溶液中FLRX和LMF浓度的增加而增加;在pH4.0～11.0条件下,FLRX、LMF分别与DNA大分子以槽沟式结合。根据实验结果利用双倒数分析法计算出FLRX、LMF分别与DNA相互作用的结合常数,其结合常数分别为K_DNA-FLRX=0.223×10⁴L/mol, K_DNA-LMF=0.290×10⁴L/mol。

关键词: 鲑鱼精DNA;紫外光谱;氟罗沙星(FLRX);洛美沙星(LMF)

Abstract:

The interaction, at different time, different temperature and different concentration, between FLRX、LMF and Salmon sperm DNA are studied by UV-visible spectrophotometry. The results showed that Salmon sperm DNA′s UV adsorption values increas with the concentration of both quinolones increasing respectively. Two quinolones bind DNA′s groove at pH4.0～11.0. The combination constants of between FLRX、LMF and Salmon sperm DNA are calculated by double reciprocal analysis,and the combination constants are KDNAFLRX=0223×104L/mol and KDNALMF=0.290×10⁴L/mol.

Key words: Salmon sperm DNA; UV spectroscopy; FLRX; LMF

张碧程1,胡林2*,刘艳辉2,张婧1. 喹诺酮药物与DNA分子相互作用的紫外光谱研究[J]. J4, 2011, 46(7): 11-16.

ZHANG Bi-cheng1, HU Lin2*, LIU Yan-hui2, ZHANG Jing1. Study on interaction of both quinolones with DNA by ultraviolet spectroscopy[J]. J4, 2011, 46(7): 11-16.

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