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山东大学学报(理学版)

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葡萄酒野生酿酒酵母的筛选及其生物特性的研究

汤晓宏1,胡文效2*,魏彦锋2,蒋锡龙2,张晶莹2,   

  1. 1.齐鲁工业大学, 山东 济南 250353; 2.山东省葡萄研究院, 山东 济南 250100;
    3.中粮君顶酒庄有限公司, 山东 蓬莱 265600
  • 收稿日期:2013-12-18 出版日期:2014-03-20 发布日期:2014-05-29
  • 通讯作者: 胡文效(1964- ),男,研究员,研究方向为生物化工. E-mail:wxhu01@sohu.com
  • 作者简介:汤晓宏(1987- ),女,硕士研究生,研究方向为发酵工程. E-mail:xiaotang19@126.com

Screening and biological characteristics studies of wide wine-making yeasts

TANG Xiao-hong1, HU Wen-xiao2*, WEI Yan-feng2, JIANG Xi-long2, ZHANG Jing-ying2, SHAO Xue-dong3   

  1. 1. Qilu University of Technology, Jinan 250353, Shandong, China;
    2. Shandong Academy of Grape, Jinan 250100, Shandong, China;
    3. Chateau Junding LTD, Penglai 265600, Shandong, China
  • Received:2013-12-18 Online:2014-03-20 Published:2014-05-29

摘要: 从蓬莱葡萄酒产区君顶酒庄霞多丽葡萄果实表皮和葡萄园土壤中分离纯化得到200个单菌落,经初筛和复筛,获得性能较好的菌株11株,对其进行26SrDNA D1/D2区序列分析、形态学观察和生理生化测定,将编号为PJ16的菌株鉴定为酵母属酿酒酵母种(Saccharomyces cerevisiae)。通过耐受性及发酵性能测试,表明菌株PJ16适合用于酿造葡萄酒。所酿葡萄酒的香气成分,如正己醇、1壬醇、2壬醇、壬醛、苯甲醛、乙酸乙酯、乙酸异戊酯、异戊酸乙酯、乳酸乙酯、乙酰苯、苯并噻唑、2乙酰基呋喃、2甲基四氢噻吩3酮和β突厥酮,浓度远高于对照组商品酵母所酿葡萄酒,体现出酿酒酵母PJ16的优良特性。

关键词: 鉴定, 耐受性, 酿酒酵母, 筛选

Abstract: 200 yeast strains were isolated from chardonnay grape skin and vineyard soil of Chateau junding in Penglai Wine Region. 11 yeast strains with excellent performance for wine were selected, one of them (PJ16) was identified as Saccharomyces cerevisiae, respectively based on the analysis of 26SrDNA D1/D2 sequence combination with morphological observation and biochemical and physiological characteristics.Through detection of tolerance and fermentation test, the results showed that the strain PJ16 is suited for brewing grape wine. And the concentrations of its aroma components produced by the strain PJ16, including hexyl alcohol, 1-nonyl alcohol, 2-nonyl alcohol, nonyl aldehyde, benzaldehyde, ethyl acetate, isoamyl acetate, isoamyl acid ethyl ester, ethyl lactate, acetophenone, benzothiazole and 2-acetyl furan, 2-methyl tetrahydrothiophene-3-ketone and beta turkic ketone, were much higher than those of the control group, suggesting that the strain PJ16 had excellent characteristics for wine-making.

Key words: Saccharomyces cerevisiae, ethanol tolerance, screening, identify

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