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《山东大学学报(理学版)》 ›› 2022, Vol. 57 ›› Issue (1): 1-7.doi: 10.6040/j.issn.1671-9352.0.2021.323

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4种鲍肠道菌群多样性比较

郭战胜,张海涛,侯旭光*   

  1. 山东大学海洋学院, 山东 威海 264209
  • 发布日期:2021-12-21
  • 作者简介:郭战胜(1987— ),男,博士研究生,研究方向为鲍遗传育种研究. E-mail:guozhansheng@sdu.edu.cn*通信作者简介:侯旭光(1963— ),男,博士,教授,研究方向为鲍遗传育种研究. E-mail:houxuguang@sdu.edu.cn
  • 基金资助:
    山东省自然科学基金资助项目(ZR2020MC195)

Comparision of the intestinal microflora diversity of four species of abalone

GUO Zhan-sheng, ZHANG Hai-tao, HOU Xu-guang*   

  1. Marine College, Shandong University, Weihai 264209, Shandong, China
  • Published:2021-12-21

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摘要： 利用16S rRNA高通量测序技术比较皱纹盘鲍、黑足鲍、黑唇鲍和杂交鲍(皱纹盘鲍♀×黑足鲍♂)菌群结构特征、多样性。结果表明,OTU数量、Shannon指数和Chao1指数均为黑足鲍最大,黑唇鲍最小;4种鲍肠道中优势菌门为变形菌门(Proteobacteria)、梭杆菌门(Fusobacteria)和软壁菌门(Tenericutes),占比达到61.93%~95.78%。其中,皱纹盘鲍、黑足鲍和杂交鲍中绝对优势菌门为变形菌门,而黑唇鲍为软壁菌门;冷泥杆属(Psychrilyobacter)、弧菌属(Vibrio)和支原体属(Mycoplasma)为4种鲍肠道的优势菌属,占比达到39.70%~53.65%;4种鲍核心菌群共有175个OTU,相对丰度超过1%的共有11个OTU;Anosim和Adonis分析均发现杂交鲍与黑唇鲍、杂交鲍与黑足鲍、皱纹盘鲍与黑唇鲍肠道菌群存在显著性差异(P<0.05); 经LefSe分析鉴定出4种鲍肠道菌群具有显著性差异的Biomarker共有7个,包括黑足鲍的6个和杂交鲍的1个。

关键词: 鲍, 高通量测序, 肠道菌群, 核心菌群

Abstract: The composition and diversity of intestinal microflora of Haliotis discus hannai, H. iris, H. rubra and the hybrid abalone(H. discus hannai ♀×H. iris ♂)were investigated using 16S rRNA high-throughput sequencing technology. The results showed that the OTU number, the values of Shannon index and Chao1 index were the highest in H. iris, and the lowest in H. rubra. The dominant phyla in the intestines of the four abalone species were Proteobacteria, Fusobacteria and Tenericutes, accounting for 61.93% to 95.78%. Among them, the absolute dominant phylum in H. discus hannai, H. iris and hybrid abalone was Proteobacteria, while that in H. rubra was Tenericutes. Psychrilyobacter, Vibrio and Mycoplasma were the predominant genera of the intestinal tract of the four species of abalone, accounting for 39.70% to 53.65%. 175 OTUs belong to the core microflora were detected in four species of abalone, a total of 11 OTUs have been found that the relative abundance exceeded 1%. Both Anosim and Adonis analyses found that the intestinal microflora differed significantly between hybrid abalone and H. rubra, hybrid abalone and H. iris, and H. discus hannai and H. rubra(P<0.05); LefSe was used to analyze the differences of microbial taxon composition among abalones and seven biomarkers were identified, including six biomarkers in H. iris and one in hybrid abalone.

Key words: abalone, high-throughput sequencing, intestinal microflora, core microflora

中图分类号: 

  • S938.8
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