利用γ-丁内酯衍生物研究空泡化与细胞分化

利用γ-丁内酯衍生物研究空泡化与细胞分化

井庆川¹，赵静²，赵宝祥^3*，张尚立²，苗俊英^2*

1. 山东省农业科学院家禽研究所，山东济南 250023；2. 山东大学生命科学学院发育研究所，山东　济南　250100；3. 山东大学化学与化工学院有机化学研究所，山东　济南　250100

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2006-10-24 发布日期:2006-10-24
通讯作者: 井庆川

The vacuolization and differentiation of vascular endothelial cells by a γ-butyrolactones derivative

JING Qing-chuan¹， ZHAO Jing²， ZHAO Bao-xiang^3*，ZHANG Shang-li²，MIAO Jun-ying^2*

1. Institute of Poultry Science, Shandong Academy of Agricultural Sciences;2. Institute of Development Biology, College of Life Science, Shandong University;3. Institute of Organic Chemistry, School of Chemistry and Chemical Engineering

Received:1900-01-01 Revised:1900-01-01 Online:2006-10-24 Published:2006-10-24
Contact: JING Qing-chuan

摘要/Abstract

摘要： 从化学遗传学的角度，利用γ-丁内酯衍生物研究了成纤维细胞生长因子（fibroblast growth factor, FGF）诱导鸡胚盘细胞向血管内皮细胞分化过程中，空泡化对血管内皮细胞分化的影响。中性红染色及吖啶橙染色结果表明，在血管内皮细胞分化过程中出现的膜泡为酸性膜泡，γ-丁内酯衍生物可能通过抑制这些酸性膜泡，抑制了鸡胚盘细胞向血管内皮细胞的分化。该研究结果进一步证实了空泡化在细胞分化中的作用，并为研究γ-丁内酯衍生物的靶蛋白在鸡胚盘细胞向内皮细胞分化中的作用打下了基础。

关键词: 鸡胚盘细胞, 空泡, γ-丁内酯衍生物 , 化学遗传学, 内皮细胞分化

Abstract: The effects of vacuolization on the differentiation of vascular endothelial cells were analyzed from a chemical biochemistry genetics perspective using the blastodisc cells differentiation model induced by FGF with the γ-butyrolactones derivative. The results of neutral red staining and acridine orange staining show that the vacuoles are acid, γ-butyrolactones derivative can suppress the differentiation of chick blastodisc cells to vascular endothelial cells by inhibiting the acid vacuoles in cells. The data validates the effect of vacuolizationon cell differentiation, which can be regarded as a reference for future research on the target protein functions of γ-butyrolactones derivative in chick blastodisc cells differentiation to endothelial cells.

Key words: γ-butyrolactones derivative , chemical genetics, differentiation of endothelial cell, vacuoles, chick blastodisc cell

中图分类号:

井庆川,赵静,赵宝祥*,张尚立,苗俊英* . 利用γ-丁内酯衍生物研究空泡化与细胞分化[J]. J4, 2008, 43(3): 6-09 .

JING Qing-chuan,ZHAO Jing,ZHAO Bao-xiang*,ZHANG Shang-li,MIAO Jun-ying* . The vacuolization and differentiation of vascular endothelial cells by a γ-butyrolactones derivative[J]. J4, 2008, 43(3): 6-09 .

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