Abstract:

The aims of this paper are to explore the optimal method of isolating, purifying, and proliferating Mongolian sheep adipose-derived mesenchymal stem cells (ADSCs), and their multiple differentiation potentiality. ADSCs were harvested by centrifuging after collagenase I digestion and obtained mononuclear cells were cultured. The results showed that, the isolated adipose mesenchymal stem cells were uniform, a fibroblast like spindle or stellate. Analysis of the growth of the passage 1, 5, and 10 cultures revealed an S-shaped growth curve with the population doubling time of 34.1 h. The P3 ADSCs were cultured in vitro under inductive environments and induced into neurogenesis and cardiomyocytes. Their differentiation properties were confirmed by histological staining such as toluidine blue, and periodic acid schiff. RT-PCR showed that the specific genes to be induced were all expressed. This proves that the isolated cells are indeed the ADSCs, and also provides valuable materials for somatic cell cloning and transgenic research.

Key words: ADSCs, differentiatio, mongolia sheep, adipose tissue