Loading...

Table of Content

      
    20 January 2014
    Volume 49 Issue 1
    Articles
    Research advances of tissue-engineered human corneal endothelium
    FAN Ting-jun, DIAO Jin-mei
    JOURNAL OF SHANDONG UNIVERSITY(NATURAL SCIENCE). 2014, 49(1):  1-7.  doi:10.6040/j.issn.1671-9352.9.2013.004
    Abstract ( 423 )   PDF (1048KB) ( 605 )   Save
    Related Articles | Metrics

    From the two aspects including sources of seeder cells, and preparation of scaffold carriers, research advances of tissue-engineered human corneal endothelium are reviewed here.

    Cytotoxic effects of lidocaine on human corneal epithelial cells and#br# its underlying mechanisms
    MIAO Ying, WANG Rui-xin, YU Hao-ze, YU Mao-miao, GE Yuan, FAN Ting-jun*
    JOURNAL OF SHANDONG UNIVERSITY(NATURAL SCIENCE). 2014, 49(1):  8-14.  doi:10.6040/j.issn.1671-9352.0.2013.489
    Abstract ( 462 )   PDF (4091KB) ( 744 )   Save
    Related Articles | Metrics

    To investigate the cytotoxic effect of lidocaine on human corneal epithelial (HCEP) cells and its underlying mechanism, in vitro cultured HCEP cells were treated with lidocaine at different doses and examined by light microscopy, MTT assay, acridine orange/ethidium bromide (AO/EB) doublefluorescent staining, DNA electrophoresis, TUNEL assay, flow cytometry, and transmission electron microscopy (TEM). Results of light microscopy and MTT assay showed that lidocaine at doses of 125~1000g/L exhibited significant cytotoxicity to HCEP cells, in a doseand timedependent manner. Results of AO/EB doublefluorescent staining showed that lidocaine at doses of 0625~10000g/L elevated the plasma membrane permeability of HCEP cells, and the apoptotic rate of lidocaine-induced HCEP cells was also in a dose-and time-dependent manner. Results of DNA electrophoresis and TUNEL assay showed that lidocaine induced DNA fragmentation of HCEP cells. TEM observation showed that lidocaine induced ultrastructural changes of HCEP cells which were similar to that of apoptotic cells, such as cytoplasmic vacuolation, chromatin condensation, disordered cristae in swollen mitochondria, presence of apoptotic bodies, etc. Flow cytometry of annexin V/PI staining showed that lidocaine induced translocation of phospholipid phosphatidylserine (PS) in plasma membranes of HCEP cells. ELISA assay showed that lidocaine induced over-expression of caspase 3,8,9,10 in HCEP cells, indicating that lidocaine could induce apoptosis of HCEP cells, not necrosis. In conclusion, lidocaine at dose above 0.625g/L has significant cytotoxicity to HCEP cells in dose-and time-dependent manners, which is realized by inducing apoptosis in these cells, suggesting the inescapable sever cytotoxic side effect of lidocaine in eye clinic which should be utilized with attention.

    Comparative studies of the nutritional compositions of Haliotis discus hannai, #br# Haliotis iris and their hybrid F1 generation
    GUO Zhan-sheng, HOU Xu-guang*,ZHANG Hai-tao, ZHENG Hai, SHI Chao,WANG Shuai, FANG Xiao-ning
    JOURNAL OF SHANDONG UNIVERSITY(NATURAL SCIENCE). 2014, 49(1):  15-19.  doi:10.6040/j.issn.1671-9352.0.2013.362
    Abstract ( 525 )   PDF (918KB) ( 887 )   Save
    Related Articles | Metrics

    The nutritional compositions, amino acid and fatty acid compositions and contents, mineral material content, the total sugar and the reducing sugar contents of the foot muscle of H.discus hannai, H.iris and their hybrids were analyzed by using biochemical methods. The results showed that the hybrids contained the rishest protein(17.97%), H.iris contained the rishest fat(1%) and sugar (9.29%). The total amino acid content(58.91%) and the deliocious amino acid content(28.77%)of the hybrids were highest, the essential amino acid content of H.iris was highest (21.33%), the contents of them in H.discus hannai foot muscle were least. The composition of fatty acids and the content the unsaturated fatty acids of in H.iris and the hybrids body were higher than H.discus hannai. In the nine testing mineral material, except the content of Mn and Cu was highest in the foot muscle of H.iris obviously, while the content of the other seven kinds of element in their foot muscles showed little differences. The total sugar and the reducing sugar contents of H.iris were the highest, and the contents of them in H.discus hannai body were the least. Based on these findings, some inherited defective qualities of the next generation can be improved by interspecific hybridization, the hybrids have some qualities that are superior to their parents and indicated heterosis.

    Isolation, culture and multiple differentiation induction of mongolia sheep#br# adipose tissue-derived mesenchymal stem cells
    GAO Jin-fang1,2, LIU Zong-zheng1,2, WEI Lin-gai1,2, ZHOU Huan-min1,2, ZHANG Yan-ru1,2﹡, CAO Jun-wei1,2
    JOURNAL OF SHANDONG UNIVERSITY(NATURAL SCIENCE). 2014, 49(1):  20-24.  doi:10.6040/j.issn.1671-9352.0.2013.409
    Abstract ( 422 )   PDF (1662KB) ( 802 )   Save
    Related Articles | Metrics

    The aims of this paper are to explore the optimal method of isolating, purifying, and proliferating Mongolian sheep adipose-derived mesenchymal stem cells (ADSCs), and their multiple differentiation potentiality. ADSCs were harvested by centrifuging after collagenase I digestion and obtained mononuclear cells were cultured. The results showed that, the isolated adipose mesenchymal stem cells were uniform, a fibroblast like spindle or stellate. Analysis of the growth of the passage 1, 5, and 10 cultures revealed an S-shaped growth curve with the population doubling time of 34.1 h. The P3 ADSCs were cultured in vitro under inductive environments and induced into neurogenesis and cardiomyocytes. Their differentiation properties were confirmed by histological staining such as toluidine blue, and periodic acid schiff. RT-PCR showed that the specific genes to be induced were all expressed. This proves that the isolated cells are indeed the ADSCs, and also provides valuable materials for somatic cell cloning and transgenic research.

    The preparation and purification of Supervillin antibody
    ZHANG Tong-tong, QIAN Ji-cheng, ZHU Chang-jun, DONG Zhi-xiong*
    JOURNAL OF SHANDONG UNIVERSITY(NATURAL SCIENCE). 2014, 49(1):  25-30.  doi:10.6040/j.issn.1671-9352.0.2013.445
    Abstract ( 434 )   PDF (1589KB) ( 749 )   Save
    Related Articles | Metrics

    Gelling spot protein Supervillin(SVIL) is a membrane protein molecule located in cells and extracellular matrix interface adhesive spots. To further study the cellular function of SVIL protein,we constructed plasmids of pGEXKG-SVILC302 and pHIS8-SVILC302 to express recombinant protein GST-SVILC302 and HIS-SVILC302 in E.coli BL21-DE3, respectively. The GST-SVILC302 protein was purified by Glutathione affinity chromatography and used to immune New Zealand white rabbits to generate the polyclonal antibody of SVIL. The HIS-SVILC302 protein was crosslinked with Ni-TNA Beads for affinity purification of the antibody. The results of Western Blot and Immunoflurescence experiments demonstrated that the antibody can specific recognize exogenous GFP-SVIL and endogenous SVIL protein. Our results showed that anti-SVIL polyclonal antibody we generated has high specificity and sensitivity enough to study the cellular functions of SVIL protein.

    Novel synthesis of C-terminal polypeptides by ISPPS method
    CHEN Lei, JIN Zhi, LIU Qiang, WANG Ming, WANG Pu*
    JOURNAL OF SHANDONG UNIVERSITY(NATURAL SCIENCE). 2014, 49(1):  31-35.  doi:10.6040/j.issn.1671-9352.0.2013.235
    Abstract ( 452 )   PDF (1456KB) ( 891 )   Save
    Related Articles | Metrics

    A facile approach for the preparation of polypeptide C-terminal derivatives by inverse solid phase peptide synthesis (ISPPS) was developed. This novel method combines both advantages of Fmoc-SPPS and TAEC technology, utilizes fewer functional group protection steps. In addition, the reaction can be carried out at room temperature under mild conditions and be easily monitored, and the working-up is simple and efficient. This method is complementary to the direct solid phase peptide synthesis. Several polypeptides as well as their C-terminal derivatives were efficiently prepared by this novel ISPPS method.

    HPLC separation of enantiomers of dracocephins A
    LI Chao, ZHAO Li-juan, ZHANG Yao, REN Dong-mei*
    JOURNAL OF SHANDONG UNIVERSITY(NATURAL SCIENCE). 2014, 49(1):  36-38.  doi:10.6040/j.issn.1671-9352.0.2013.419
    Abstract ( 415 )   PDF (1255KB) ( 677 )   Save
    Related Articles | Metrics

    To establish a direct enantioseparation method by HPLC on immobilized amylose-based chiral stationary phase for the chiral separation of the enantiomers of dracocephins A. The separation was carried out using a Chiralpak IA column (4.6mm×250mm, 5μm) in combination with n-hexane-ethyl acetate (10∶90) as mobile phase. The flow rate was 1.0mL·min-1 and detection wavelength was 290nm, whilst the column temperature was room temperature. The established chromatographic method was effective for the simultaneous separation of the two pairs of enantiomers of dracocephins A. The elution order was determined by coupling HPLC-CD detection and comparison with each single enantiomer. Immobilized amylosebased chiral stationary phase is suitable for the enantioseparation of dracocephins A.

    Correlation analysis between cerebral oxygen and arterial #br# blood pressure signals based on cross wavelet analysis
    CUI Ruo-fei, LU Li-qian, ZHOU Wei-wei, LI Wen-hao, HAN Qing-yu, GAO Yuan-jin, LI Zeng-yong*
    JOURNAL OF SHANDONG UNIVERSITY(NATURAL SCIENCE). 2014, 49(1):  39-43.  doi:10.6040/j.issn.1671-9352.0.2013.256
    Abstract ( 385 )   PDF (3162KB) ( 616 )   Save
    Related Articles | Metrics

    The objective of this study was to assess the correlation between spontaneous oscillations in Nearinfrared cerebral oxygenation (CO) and arterial blood pressure (ABP) and to propose a method of indicating brain stroke. Continuous recordings of NIRS and ABP signals were obtained from simultaneous measurements in nineteen young subjects (age=24±2 years), thirty two old subjects (age=70.8±5.2 years) at rest. The correlations between CO signals and ABP signals were analyzed using method based on Cross-wavelet Coherence. High common power can be observed in timefrequency domain between the two signals. What′s more, the coherency shows that there are significant relationships as well in interval I and interval II. Significant difference can be observed (P=0.002, F=11.215) after statistical analyzing the coherence between CO signals and ABP signals of the three groups in interval I. The results indicated that comparing with the young the coherence of the frequency reflecting cardiac activity between CO signals and ABP signals became higher, which may signify high stiffness of cerebral vascular.

    Construction and application of SSR fingerprints of hybrid #br# cotton variety Lu 05H9 with red-corolla marker trait
    ZHAO Jun-sheng1,2, WANG Xiu-li1,2, GAO Ming-wei1,2, WANG Jia-bao1,2, WANG Yong-cui1,2, CHEN Ying1,2, JIANG Hui1,2, YANG Jing1,2, WANG Liu-mi
    JOURNAL OF SHANDONG UNIVERSITY(NATURAL SCIENCE). 2014, 49(1):  44-49.  doi:10.6040/j.issn.1671-9352.0.2013.286
    Abstract ( 421 )   PDF (2028KB) ( 521 )   Save
    Related Articles | Metrics

    SSR markers were used to analyze the fingerprinting of hybrid cotton variety Lu 05H9 with red-corolla marker trait. Lu 05H9 and its parents were amplified with 98 pairs of core primers with high polymorphisms and good repeatability. Out of them, 12 paris were detected to be polymorphic between the two parents, which were codominant markers since they amplified the different fragments between the two parents and heterozygosis bands in F1. Fingerprints of Lu05H9 were constructed with the amplification result of the 12 paris primers. It provides a method for seed genuineness and purity detection of hybrids. Due to its fast readability and large storage capacity, DNA fingerprinting of Lu05H9 is in favor of commercial applications.

    rediction of chromatograph retention index by artificial neural  network by #br# study on volatile constituents of wild chinese chives
    DU Xi-hua, SHI Xiao-qin, FENG Chang-jun, LI Liang
    JOURNAL OF SHANDONG UNIVERSITY(NATURAL SCIENCE). 2014, 49(1):  50-53.  doi:10.6040/j.issn.1671-9352.0.2013.305
    Abstract ( 408 )   PDF (933KB) ( 662 )   Save
    Related Articles | Metrics

    In order to study on the features of aromatic composition in wild Chinese chives and predict its chromatographic retention index of volatile constituents molecules, self-designed programs are run in MATLAB to get the molecular shape index and electrotopological state index of volatile constituents of wild Chinese chives. The two indexes are used as molecular structure parameters, and by using multiple stepwise regression method, we screen and optimize the structure parameters 2K、3K、4K、I2 and I6 to establish a QSRR model of chromatographic retention index of volatile constituents of wild Chinese chives. The correlation coefficient of regression equation reaches 0.963. By examining the stability and predictive ability of the model, we find the correlation coefficient r of the inspection is stable at around 0.963. The five structural parameters are used as the input neurons of the artificial neural network and a 521 network architecture is employed. A satisfying neural network model is constructed with the back-propagation(BP) algorithm. The gross correlation coefficient R is 0. 996,which shows a significant correlation. The forecasted values is basically tally with experimental values that we get from the model with only 1.67% mean relative error. It can be seen that the results of back-propagation network are better than those of multiple linear regression methods.

    Experimental investigation on the relationship between steering coefficient #br# and volume fraction in two-dimensional static granular system
    GAO Li-ke, HU Lin*, ZHANG Xing-gang, DENG Xiong
    JOURNAL OF SHANDONG UNIVERSITY(NATURAL SCIENCE). 2014, 49(1):  54-58.  doi:10.6040/j.issn.1671-9352.0.2013.475
    Abstract ( 513 )   PDF (2181KB) ( 481 )   Save
    Related Articles | Metrics

     Experimental investigation on the relationship between steering coefficient and volume fraction in two-dimensional static granular system was carried. The results show that granular volume fraction increases, steering coefficient decreases gradually and reaches a saturated state while putting a steady stress on the grains continually; at the same time, steering coefficient increases and reaches a saturated state with the increasing of granular stacking angel. According to the experimental results, a parameter equation matching with the experimental data was provided.

    SnO molecular structure and properties under external electric field
    HUANG Duo-hui, WANG Fan-hou, YUAN Di
    JOURNAL OF SHANDONG UNIVERSITY(NATURAL SCIENCE). 2014, 49(1):  59-64.  doi:10.6040/j.issn.1671-9352.0.2013.371
    Abstract ( 363 )   PDF (1468KB) ( 580 )   Save
    Related Articles | Metrics

    The structure of SnO ground state molecule is optimized by employing density functional theory (B3P86) method with SDB-cc-pVTZ basis sets for Sn atom and 6.311++G** for O atom. The effects of electric filed on the bond length, total energy, mulliken atomic charges, the Highest Occupied Molecular Orbital(HOMO) energy level, the Lowest Unoccupied Molecular Orbital(LUMO) energy level, energy gap, electric dipole moment, harmonic frequency and infrared intensity of SnO ground state molecule are studied. The excited properties of SnO molecule under different electric fields are also investigated by using configuration interaction singles density functional theory (CIS-B3P86) method. The results show that the bond length and electric dipole moment are found to firstly decrease, then increasing with increasing of positive direction electric fields, the minimums are 0.1796nm at F=0.04a.u. and 0.2124 Debye at F=0.03a.u., respectively. The total energy is proved to firstly increase, then decreasing with increasing of positive direction electric fields, the maximum is -2144.8396eV at F=0.03a.u.. The EH and harmonic frequency are found to increase with increasing of positive direction electric fields, but the EL, Eg and infrared intensity are decreasing. The magnitude and direction of the external electric field have important effects on excitation energies, oscillator strengths and the wavelength.

    Fast stochastic consensus of leader-following multi-agent network
    Shu-ling1, SHI Hong-jun2, LIU Feng2
    JOURNAL OF SHANDONG UNIVERSITY(NATURAL SCIENCE). 2014, 49(1):  65-70.  doi:10.6040/j.issn.1671-9352.0.2013.309
    Abstract ( 345 )   PDF (1365KB) ( 1168 )   Save
    Related Articles | Metrics

    By using the finite-time stability theory of stochastic differential equations and adding a simple protocol, it was proved that the leader-following multi-agent network can reach the consensus in finite time with probability one. The effects of control parameters and noise strength on the convergence speed and time were also analyzed. Furthermore, numerical examples were provided to illustrate the effectiveness of the theoretical results.

    Distributed associative classification algorithm based on improved FP-tree
    LU Qi-bei1,2, GUO Fei-peng3
    JOURNAL OF SHANDONG UNIVERSITY(NATURAL SCIENCE). 2014, 49(1):  71-75.  doi:10.6040/j.issn.1671-9352.1.2013.221
    Abstract ( 396 )   PDF (1762KB) ( 878 )   Save
    Related Articles | Metrics

    Traditional information mining technology has been unable to meet the increasingly complex application requirements in the big data environment. The distributed data mining technique is a means to solve this problem. An improved distributed associative classification algorithm based on improved FP-tree was presented. First, FP-Tree was optimized in each local node to generate local conditional pattern tree (CFP-Tree), and then a global CFP-Tree was constructed through the inter-site transmission of each CFP-Tree. Second, the initial global significant classification rules were obtained by calculating significant degree in the process of global CFP-Tree mining. Final, the pruning strategies were used to get a small set of rules to construct the overall associative classifier. Experimental results show that this algorithm can not only effectively reduce network traffic and improve mining efficiency, but also ensure ensuring statistical significance of rules and improve the ability for the discovery of implicit rules.

    Recognition method of Vietnamese named entity based on#br# conditional random fields
    PAN Qing-qing, ZHOU Feng, YU Zheng-tao, GUO Jian-yi, XIAN Yan-tuan
    JOURNAL OF SHANDONG UNIVERSITY(NATURAL SCIENCE). 2014, 49(1):  76-79.  doi:10.6040/j.issn.1671-9352.1.2013.213
    Abstract ( 529 )   PDF (1407KB) ( 653 )   Save
    Related Articles | Metrics

    A method of named entity recognition is proposed based on conditional random fields model aimed at the language feature of Vietnamese. This method aims at the feature of word and part of speech, adopts the arithmetic of conditional random fields, selects the word and part of speech as the feature, defines the feature template, chooses the news text of Vietnamese, tags the six entity linguistic data such as place name, person name and organization, trains the Vietnamese entity recognition model which acquired. Vietnamese entity recognition experiment results prove that the entity recognition accuracy rate of this method reach 83.73%.

    Stability analysis of generalized fuzzy bidirectional associative #br# memory networks with thresholds
    CHEN Gui-ying
    JOURNAL OF SHANDONG UNIVERSITY(NATURAL SCIENCE). 2014, 49(1):  80-85.  doi:10.6040/j.issn.1671-9352.0.2013.171
    Abstract ( 377 )   PDF (940KB) ( 459 )   Save
    Related Articles | Metrics

    Based on the fuzzy operator “∨” and a tnorm “T”, a generalized dynamical model named the fuzzy bidirectional associative memory networks with thresholds was set up. It is shown that the equilibrium of the system is Lyapunov stable if T satisfies Lipschitz condition. A sufficient condition for the system to be structurally stable was also proposed. Finally, an example was given to demonstrate our conclusion.

    Global behavior of solutions for a modified LeslieGower #br# predator-prey system with diffusion
    ZHANG Li-na, WU Shou-yan
    JOURNAL OF SHANDONG UNIVERSITY(NATURAL SCIENCE). 2014, 49(1):  86-91.  doi:10.6040/j.issn.1671-9352.0.2013.320
    Abstract ( 395 )   PDF (930KB) ( 492 )   Save
    Related Articles | Metrics

    The global behavior of solutions for a modified Leslie-Gower predator-prey system with diffusion is discussed. It is found that the dynamical behavior of the reaction-diffusion system is similar to that of the corresponding ordinary differential system. Therefore, Turing instability can not occur for this reactiondiffusion system.

    Asymptotic ruin probabilities of a risk model with #br# double investment strategies
    XU Tian-ming1, WU Qing-tai2*
    JOURNAL OF SHANDONG UNIVERSITY(NATURAL SCIENCE). 2014, 49(1):  92-98.  doi:10.6040/j.issn.1671-9352.0.2013.177
    Abstract ( 303 )   PDF (939KB) ( 564 )   Save
    Related Articles | Metrics

    Insurance companies adopt different strategies under different levels of surplus.The expression of the surplus process was obtained by It formula and riskneutral assumption, and then assuming the claims process belongs to D, and are pair-wise quasi-asymptotically independent. Finally the asymptotic estimations of ruin probability in finite-time and infinite-time are obtained and the corresponding numerical simulation has carried on.

    Extremal distribution for the discrete 5-convex stochastic #br# ordering and applications
    TIAN You-gong1, JIAO Gui-mei2*
    JOURNAL OF SHANDONG UNIVERSITY(NATURAL SCIENCE). 2014, 49(1):  99-104.  doi:10.6040/j.issn.1671-9352.0.2013.248
    Abstract ( 298 )   PDF (934KB) ( 544 )   Save
    Related Articles | Metrics

    The extremal distribution for the discrete 5-convex order was obtained. In applications,  the lower and upper bounds for the probability of extinction in a branching process and the Lundberg′s adjustment coefficient in the classical discrete risk model were studied.

    The r-th rate of consistency in kernel density estimation for #br# φ-mixed random censored samples
    WU Xin-ye, WU Qun-ying
    JOURNAL OF SHANDONG UNIVERSITY(NATURAL SCIENCE). 2014, 49(1):  105-110.  doi:10.6040/j.issn.1671-9352.0.2013.308
    Abstract ( 434 )   PDF (937KB) ( 579 )   Save
    Related Articles | Metrics

    The r-th rate of consistency of the Kaplan-Meier estimation fn(x) for a density function f(x) based on the censored data was obtained when the survival and censoring times form a stationary φ-mixed sequence.