JOURNAL OF SHANDONG UNIVERSITY(NATURAL SCIENCE) ›› 2015, Vol. 50 ›› Issue (01): 56-61.doi: 10.6040/j.issn.1671-9352.0.2014.330

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Spectroscopic study on the interaction between nC60 nanoparticles and bovine hemoglobin

YAO Yao, LIN Nan-yu, LIU Shu-fang   

  1. School of Public Health, Shandong University, Jinan 250012, Shandong, China
  • Received:2014-07-10 Revised:2014-10-29 Online:2015-01-20 Published:2015-01-24

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Abstract: Several spectroscopic methods were used to study the interaction between nC60 nanoparticles and bovine hemoglobin. The nC60 nanoparticles dispersion in water was prepared by solvent replacement method and characterized using UV-Vis spectrometry, dynamic light scattering and transmission electron microscopy. The interaction between nC60 and bovine hemoglobin was investigated by UV-Vis spectrometry, fluorescence spectroscopy and synchronous fluorescence spectroscopy. The result indicated that the UV-Visible absorption spectrum of bovine hemoglobin was changed to some extent by nC60 nanoparticles. The intrinsic fluorescence of bovine hemoglobin could be quenched by nC60 and the maximum emission wavelength showed obvious blue-shift. With the increasing concentrations of nC60, the synchronous fluorescence intensity of tyrosine and tryptophan residues decreased gradually, and the fluorescence peak of tyrosine residues exhibited a blue shift while the fluorescence peak of tryptophan residues remained unchanged. The research showed that the binding of nC60to bovine hemoglobin could cause the conformation change of bovine hemoglobin. The microenvironment polarity around tyrosine residues decreased, indicating the binding site of nC60 to bovine hemoglobin was located near tyrosine residues.

Key words: fullerene, fluorescence, C60, bovine hemoglobin

CLC Number: 

  • O657.3
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