JOURNAL OF SHANDONG UNIVERSITY(NATURAL SCIENCE) ›› 2015, Vol. 50 ›› Issue (11): 1-7.doi: 10.6040/j.issn.1671-9352.0.2014.376

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Chromokinesin KIF4A inhibits migration of gastric cancer cells

HU Xiao-qing1,3, LIANG Shuang-shuang1,3, JI Mei-chao1,3, ZHU Chang-jun1,2,3, PAN Li-na1,2,3   

  1. 1. College of Life Science, Tianjin Normal University, Tianjin 300387, China;
    2. Tianjin Key Laboratory of Animal and Plant Resistance, Tianjin 300387, China;
    3. Key Laboratory of Molecular and Cellular Systems Biology, Tianjin Normal University, Tianjin 300387, China
  • Received:2014-08-20 Revised:2015-01-15 Online:2015-11-20 Published:2015-12-09

Abstract: To explore the role of chromokinesin KIF4A regulating migration of gastric cancer cells and then provide a potential therapeutic targets for clinical treatment of gastric cancer. Gastric cancer cell lines were constructed with either stably lower-expression or stably over-expression of KIF4A using G418 selection. Then we verified these cell lines using Western Bolt and immunofluorescent staining. These cells' migration abilities were assayed with wound healing and Transwell experiments. KIF4A stably lower-expression (SGC-shKIF4A) or overexpression cell lines (SGC-KIF4A) were successfully constructed and cell migration of SGC-shKIF4A cells were faster than that of SGC-KIF4A cell lines. Chromokinesin KIF4A inhibits the ability of gastric cancer cell movement laying a foundation for studies of KIF4A's roles in gastric cancer metastasis.

Key words: chromokinesin, Gastric cancer, cell migration, KIF4A

CLC Number: 

  • Q78
[1] LIN Wenli, SUN Jialing, CHANG Shuchan, et al. Factors predicting survival of patients with gastric cancer[J]. Asian Pac J Cancer Prev, 2014, 15(14):5835-5838.
[2] SEHDEV A, CATENACCI D V. Gastroesophageal cancer:focus on epidemiology, classification, and staging[J]. Discov Med, 2013, 16(87):103-111.
[3] FRIDOLFSSON H N, STARR D A. Kinesin-1 and dynein at the nuclear envelope mediate the bidirectional migrations of nuclei[J]. J Cell Biol, 2010, 191(1):115-128.
[4] THEISEN U, STRAUBE E, STRAUBE A. Directional persistence of migrating cells requires Kif1C-mediated stabilization of trailing adhesions[J]. Dev Cell, 2012, 23(6):1153-1166.
[5] CORNFINE S, HIMMEL M, KOPP P, et al. The kinesin KIF9 and reggie/flotillin proteins regulate matrix degradation by macrophage podosomes[J]. Mol Biol Cell, 2011, 22(2):202-215.
[6] AHMED S M, THERIAULT B L, UPPALAPATI M, et al. KIF14 negatively regulates Rap1a-Radil signaling during breast cancer progression[J]. J Cell Biol, 2012, 199(6):951-967.
[7] SAMEJIMA K, SAMEJIMA I, VAGNARELLI P, et al. Mitotic chromosomes are compacted laterally by KIF4 and condensin and axially by topoisomerase IIalpha[J]. J Cell Biol, 2012, 199(5):755-770.
[8] MAZUMDAR M, SUNDARESHAN S, MISTELI T. Human chromokinesin KIF4A functions in chromosome condensation and segregation[J]. J Cell Biol, 2004, 166(5):613-620.
[9] DIKOVSKAYA D, KHOUDOLI G, NEWTON I P, et al. The adenomatous polyposis coli protein contributes to normal compaction of mitotic chromatin[J]. PLoS One, 2012, 7(6):e38102.
[10] MAZUMDAR M, SUNG M H, MISTELI T. Chromatin maintenance by a molecular motor protein[J]. Nucleus, 2011, 2(6):591-600.
[11] WANDKE C, BARISIC M, SIGL R, et al. Human chromokinesins promote chromosome congression and spindle microtubule dynamics during mitosis[J]. J Cell Biol, 2012, 198(5):847-863.
[12] ZHU Changjun, LAU E, SCHWARZENBACHER R. Spatiotemporal control of spindle midzone formation by PRC1 in human cells[J]. Proc Natl Acad Sci U S A, 2006, 103(16):6196-6201.
[13] KURASAWA Y, EARNSHAW W C, MOCHIZUKI Y. Essential roles of KIF4 and its binding partner PRC1 in organized central spindle midzone formation[J]. EMBO J, 2004, 23(16):3237-3248.
[14] ZHU Changjun, JIANG Wei. Cell cycle-dependent translocation of PRC1 on the spindle by Kif4 is essential for midzone formation and cytokinesis[J]. Proc Natl Acad Sci U S A, 2005, 102(2):343-348.
[15] HU Chikuo, COUGHLIN M, FIELD C M. KIF4 regulates midzone length during cytokinesis[J]. Curr Biol, 2011, 21(10):815-824.
[16] WU Guikai, ZHOU Longen, KHIDR L, et al. A novel role of the chromokinesin Kif4A in DNA damage response[J]. Cell Cycle, 2008, 7(13):2013-2020.
[17] BERNASCONI P, CAPPELLETTI C, NAVONE F, et al. The kinesin superfamily motor protein KIF4 is associated with immune cell activation in idiopathic inflammatory myopathies[J]. J Neuropathol Exp Neurol, 2008, 67(6):624-632.
[18] GAO Zhiguang, COX J L, GILMORE J M, et al. Determination of protein interactome of transcription factor Sox2 in embryonic stem cells engineered for inducible expression of four reprogramming factors[J]. J Biol Chem, 2012, 287(14):11384-11397.
[19] 刘海燕,李培,高辉,等. 染色体驱动蛋白KIF4A对胃癌细胞迁移和转移的影响[J]. 山东大学学报:医学版, 2013,51(12):1-4. LIU Haiyan, LI Pei, GAO Hui, et al. Chromosome kinesin KIF4A effects on gastric cancer cell migration and metastasis[J]. Journal of Shandong university:medical Science, 2013, 51(12):1-4.
[20] 钟铠泽,赵建,李培,等. 染色体驱动蛋白KIF4A稳定地表达胃癌细胞系的构建和鉴定[J]. 国际生物医学工程杂志,2014,37(1):22-25. ZHONG Kaize, ZHAO Jian, LI Pei, et al. Construction and identification of chromokinesin KIF4A knockdown gastric cancer cell lines[J]. Int J Biomed Eng, 2014, 37(1):22-25.
[21] NUNES BASTOS R, GANDHI S R, BARON R D. Aurora B suppresses microtubule dynamics and limits central spindle size by locally activating KIF4A[J]. J Cell Biol, 2013, 202(4):605-621.
[22] FEDECHKIN S O, BROCKERMAN J, LUNA E J. An N-terminal, 830 residues intrinsically disordered region of the cytoskeleton-regulatory protein supervillin contains Myosin II- and F-actin-binding sites[J]. J Biomol Struct Dyn, 2013, 31(10):1150-1159.
[23] 张佟佟,钱积成,朱长军,等. 黏着斑蛋白Supervillin的抗体制备及纯化[J]. 山东大学学报:理学版,2014,49(1):25-30. ZHANG Tongtong, QIAN Jicheng, ZHU Changjun, et al. The preparation and purification of Supervillin antibody[J]. Journal of Shandong University:Natural Science, 2014, 49(1):25-30.
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