JOURNAL OF SHANDONG UNIVERSITY(NATURAL SCIENCE) ›› 2015, Vol. 50 ›› Issue (01): 37-41.doi: 10.6040/j.issn.1671-9352.0.2014.412

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Degradation of co-activator Yki of the hippo pathway through ubiquitinationby Slimb

GUO JI-wei, JIN Dan   

  1. School of Life Sciences, Nankai University, Tianjin 300071, China
  • Received:2014-09-17 Revised:2014-11-07 Online:2015-01-20 Published:2015-01-24

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Abstract: Yki, an important co-transcription factor, regulates the expression of downstream target genes of the hippo pathway. Yki is known to be important for development, growth, organogenesis and maintenance of adult stem cells. Slimb as a substrate specificity subunit of the SCF E3 ubiquitin ligase promotes proteins for degradation in the drosophila. In this paper, we found that Slimb degraded Yki by the ubiquitination and the co-immunoprecipitation indicated that Slimb interacted with Yki in vitro. Furthermore, we found that co-overexpression with Slimb caused mostly suppressed Yki wing phenotype and knockdown of Slimb enhanced overexpression with Yki eye phenotype. These results suggested that Slimb as a subunit of the E3 ubiquitin ligase regulated the stability of Yki and provided an important significance in the regulating tissue growth and development of hippo pathway.

Key words: hippo pathway, yki, ubiquitination, degradation, slimb

CLC Number: 

  • Q257
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