JOURNAL OF SHANDONG UNIVERSITY(NATURAL SCIENCE) ›› 2015, Vol. 50 ›› Issue (11): 16-22.doi: 10.6040/j.issn.1671-9352.0.2014.543

Previous Articles     Next Articles

Expression of thioredoxin gene in sea cucumber (Apostichopus japonicus) stressed by Vibrio splendidus and V.anguillarum

LV Xin-fang1, KONG Ling-ming1, WANG Xue-guang1, ZHANG Xiao-yu1, WANG Wen-quan2, DU Zong-jun1, LI Yu-chun1   

  1. 1. Marine College, Shandong University at Weihai, Weihai 264209, Shandong, China;
    2. Institute of Tropical Biosciences and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, Hainan, China
  • Received:2014-12-01 Revised:2015-02-27 Online:2015-11-20 Published:2015-12-09

PDF (PC)

663

Abstract: Expressions of Thioredoxin (Trx) gene were analyzed by quantitative real-time PCR method in the body wall, intestine, respiratory tree, tentacles and coelomocyte of sea cucumber (Apostichopus japonicus) injected with Vibrio splendidus and V.anguillarum for 0 h, 3 h, 6 h, 9 h, 12 h, 24 h to explore the role of Thioredoxin protein in the innate immunity of sea cucumber. The Trx mRNA were identified by using quantitative real-time PCR in different tissues of sea cucumbers including the body wall, intestine, respiratory tree, tentacles and coelomocyte. Expression patterns of Trx in different tissues were similar in response to Vibrio splendidus, which presented a increase-discrease-increase model. Moreover, expressions of Trx after V.anguillarum challeng in ditected tissues seemed different in reaction time and increased speed. These results suggested that expression of Trx presented constitutive and inducible patterns in all the detected tissues, and it was tissue-specific and pathogen-specific expression in the sea cucumber. Thus, Trx may play a very important role in resisting exogenous bacteria and balancing the cellular redox state.

Key words: Apostichopus japonicus, Thioredoxin (Trx), Vibrio splendidus, Vibrio anguillarum, Innate Immunity, Tissue Expression Analysis

CLC Number: 

  • Q178.53
[1] 牛桂军, 唐国都. 硫氧还蛋白1与炎性相关性疾病[J]. 医学综述,2010, 16(6):808-811. NIU Guijun, TANG Guodu. Thioredoxin-1 and inflammation associated diseases[J]. Medical Recapitulate, 2010, 16(6):808-811.
[2] 廖玉麟. 我国的海参[J]. 生物学通报,2001, 35(9):1-3. LIAO Yulin. Sea cucumber in China[J]. Bulletin of Biology, 2001, 35(9):1-3.
[3] 杜秀敏, 殷文璇, 赵彦修,等. 植物中活性氧的产生及清除机制[J]. 生物工程学报,2001, 17(2):121-125. DU Xiumin, YIN Wenxuan, ZHAO Yanxiu, et al. The production and scavenging of reactive oxygen species in plants[J]. Chinese Journal of Biotechnology, 2001, 17(2):121-125.
[4] 田燚, 马增艳, 常亚青. 仿刺参超氧化物歧化酶基因cDNA全序列的克隆及其特征分析[J]. 渔业科学进展,2011, 32(5):97-107. TIAN Yi, MA Zengyan, CHANG Yaqing. Cloning and sequence analysis of superoxide dismutase gene in sea cucumber Apostichopus japonicus (Selenka)[J]. Progress in Fishery Sciences, 2011, 32(5):97-107.
[5] 王清印. 从产量到质量——海水养殖业发展的必然趋势[M]. 北京:海洋出版社, 2009.
[6] 张春云, 王印庚, 荣小军. 养殖刺参腐皮综合征病原菌的分离与鉴定[J].水产学报,2006, 30(1):118-123. ZHANG Chunyun, WANG Yingeng, RONG xiaojun. Isolation and identification of causative pathogen for skin ulcerative syndrome in Apostichopus japonicus[J]. Journal of Fisheries of China, 2006, 30(1):118-123.
[7] 刘智超. 养殖大菱鲆4种致病菌多重PCR和荧光定量PCR检测方法的建立[D]. 青岛:中国海洋大学生命科学学院, 2012.
[8] 王义鹏,赖仞. 昆虫抗菌肽结构、性质和基因调控[J]. 动物学研究,2010,31(1):27-34. WANG Yipeng, LAI Ren. Insect antimicrobial peptides:structures,properties and gene regulation[J]. Zoological Research, 2010, 31(1):27-34.
[9] MATSUZAWA A, NISHITOH H, TOBIUME K,et al. Physiological roles of ASK1-mediated signal transduction in oxidative stress- and endoplasmic reticulum stress-induced apoptosis:Advanced findings from ASK1 knockout mice[J]. Antioxidants & Redox Signaling,2002, 4(3):415-425.
[10] ROOS W P, KAINA B. DNA damage-induced cell death by apoptosis[J]. Trends in Molecular Medicine, 2006, 12(9):440-450.
[11] 段莹亮, 白洁. 硫氧还蛋白(Trx)及Trx80在免疫系统中的作用[J]. 免疫学杂志,2009, 25(4):483-486. DUAN Yingliang, BAI Jie. Roles of thioredoxin and Trx80 in immune system[J]. Journal of Immunology, 2009, 25(4):483-486.
[12] KIM D H, KIM J W, JEONG J M,et al. Molecular cloning and expression analysis of a thioredoxin from rock bream, Oplegnathus fasciatus, and biological activity of the recombinant protein[J]. Fish & shellfish immunology, 2011, 31(1):22-28.
[13] 母昌考. 中华绒螯蟹(Eriocheir Sinensis) Peroxiredoxin 6和Thioredoxin 1基因的克隆及表达[D]. 青岛:中国科学院研究生院(海洋研究所), 2009.
[14] 刘莹莹. 松江鲈(Trachidermus fasciatus)抗氧化相关基因的克隆、表达与功能分析[D]. 济南:山东大学生命科学学院, 2012.
No related articles found!
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Copyright 2018 © Editorial office of JOURNAL OF SHANDONG UNIVERSITY(NATURAL SCIENCE)
Supported by Beijing Magtech Co.Ltd