JOURNAL OF SHANDONG UNIVERSITY(NATURAL SCIENCE) ›› 2016, Vol. 51 ›› Issue (5): 18-28.doi: 10.6040/j.issn.1671-9352.0.2015.214

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Effects of simulated nitrogen deposition on soil microbial biomass and community structure in a young plantation of Castanopsis hystrix

HONG Pi-zheng1, LIU Shi-rong1*, YU Hao-long2, HAO Jian2   

  1. 1. Key Laboratory of Forest Ecology and Environmental Sciences of State Forestry Administration, Institute of Forest Ecology, Environment and Protection, Chinese Academy of Forestry, Beijing 100091, China;
    2. Experimental Center of Tropical Forestry, Chinese Academy of Forestry, Pingxiang 532600, Guangxi, China
  • Received:2015-05-05 Online:2016-05-20 Published:2016-05-16

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Abstract: By using fumigation-extraction method and phospholipid fatty acid technique, the effects of simulated nitrogen deposition on soil microbial biomass and community structure in a young Castanopsis hystrix plantation of subtropical China were determined through a field N addition experiment. Nitrogen was added at rate of 0 kg/(hm2·year)(Control), 50 kg/(hm2·year)(Low-N), 100 kg/(hm2·year)(Medium-N)and 150 kg/(hm2·year)(High-N), respectively for 17 months. The main results were as follows: Medium-N and High-N treatments significantly increased soil N availability, but significantly decreased soil pH in the test plantation. In addition, all the N addition treatments did not significantly influenced the content of SOC, while Medium-N and High-N treatments significantly decreased the 山 东 大 学 学 报 (理 学 版)第51卷 - 第5期洪丕征,等:模拟氮沉降对红椎人工幼龄林土壤微生物生物量和微生物群落结构的影响 \=-ratio of soil C/N, but significantly increased the content of soil extractable DOC. Medium-N and High-N treatments significantly decreased the content of MBC, but significantly increased the content of MBN compared with the control plots. Moreover, Medium-N and High-N treatments significantly decreased soil total PLFAs, fungal PLFAs, and bacterial PLFAs. Besides, all the N addition treatments significantly decreased the relative abundance of gram-negative bacterial PLFAs, but significantly increased the relative abundance of actinomycetes PLFAs. However, only High-N treatment significantly decreased the relative abundance of gram-positive bacterial PLFAs. In addition, Medium-N and High-N addition treatments significantly increased the biomass ratio of fungal to bacterial. We also found that N addition significantly affected soil microbial community structure through PCA analysis of 27 individual PLFAs detected in the test soils. Moreover, the redundancy analysis(RDA)demonstrated that the shift of soil microbial community structure under simulated N deposition was significantly correlated to the variations in MBN and soil pH. These results suggest that nitrogen deposition could significantly influence the stability of soil microbial community and thus negatively affect soil health and fertility in the young plantation of C.hystrix.

Key words: Young plantation, south subtropical China, simulated nitrogen deposition, soil microbial community structure, soil microbial biomass

CLC Number: 

  • S154.3
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