Table of Content

  

20 May 2016
Volume 51 Issue 5

Effects of extraction agents on the antioxidant activity and condensability of Aronia melanocarpa polyphenol

LI Zhao, LI Hong-qiang, CHEN Guo-gang, WU Jian, XU Jian

JOURNAL OF SHANDONG UNIVERSITY(NATURAL SCIENCE). 2016, 51(5):  1-5.  doi:10.6040/j.issn.1671-9352.0.2015.542

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Since the healthy effects of polyphenols have been gradually recognized, Aronia melanocarpa, a polyphenols-rich berry, received more and more attentions. Efficient extraction of total polyphenols from this kind of berry was an important way of the high-value usage. In this paper, a comparative study of extracting effects of acidic ethanol, ethanol and water on Aronia melanocarpa polyphenols was performed. The results showed that acidic ethanol had the highest polyphenols extraction ratio, while the extraction ratio of water was 28.77% of acidic ethanol. The analysis of polyphenols antioxidant activity(DPPH radical removing capability)showed that the water-extracted polyphenols had a higher antioxidant activity than those extracted from alcohol and acidic ethanol. The ratio of total DPPH radical removal capability of acidic ethanol-, ethanol- and water-extracted polyphenols was 100∶76.10∶70.83. In addition, the condensabilities of the three kinds of extractions were compared. When the extractions were evaporated to 10% of original volume, the antioxidant activity was almost not lost. However, after being freeze-dried, only the water-extractd polyphenol was 山 东 大 学 学 报 (理 学 版)第51卷 - 第5期李曌,等:野樱莓多酚提取剂对多酚抗氧化活性和可浓缩性的影响 \=-completely reconstituted. Considering the cost, safety and other factors, water had certain advantages to Aronia melanocarpa polyphenols extraction.

Reliability analysis of dental implantation and split-root technique joint repaired postoperative dental

CHEN Ling, MEN Yu-tao, WANG Jia-jiang

JOURNAL OF SHANDONG UNIVERSITY(NATURAL SCIENCE). 2016, 51(5):  6-10.  doi:10.6040/j.issn.1671-9352.0.2015.615

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Obtaining experimental models by the method of model reverse.In order to Study on reliability of dental implantation and split-root technique joint repaired postoperative dental, analysis of factors which affect the success rate of treatment, and calculating the limit load when reliability reached 80%, combining with the reliability theory to obtain the desired number of samples test and performing finite element simulation and analysis to the model. Numerical simulations show that the stress direction and bone properties are the most important factors to influence the long-term success rate of treatment,and after treatment the tooth can be competent for the daily chewing and biting tasks, but should try to avoid oblique stress and bite hard food.

Cytotoxicity of ofloxacin to human corneal epithelial cells and its cellular and molecular mechanisms

WANG De-ping, FAN Wen-yi, WEN Qian, FAN Ting-jun

JOURNAL OF SHANDONG UNIVERSITY(NATURAL SCIENCE). 2016, 51(5):  11-17.  doi:10.6040/j.issn.1671-9352.0.2015.635

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To define the cytotoxicity of ofloxacin(OFX), a widely used antibiotic drug in eye clinic, to human corneal epithelium and the possible cellular and molecular mechanisms, the cytotoxic effect, apoptosis-inducing effect and apoptosis-triggering pathways of OFX were investigated using an in vitro model of HCEP cells in this study. After in vitro cultured HCEP cells were treated with different concentrations of OFX for different time, the morphology, cytopathic effect(CPE)and viability were assessed by light microscopy and MTT assay, the cell cycle arrest and apoptosis were detected by flow cytometry(FCM), AO/EB double-staining, gel electrophoresis and transmission electron microscopy, and caspase activation, cytoplasmic amount of mitochondrion-specific apoptosis-triggering proteins and mitochondrial transmembrane potential(MTP)disruption were quantified using ELISA, Western blot and FCM. Our results revealed that OFX above concentrations of 0.375 g/L induced morphological abnormality, CPE formation, viability decline and plasma membrane permeability elevation of HCEP cells in a dose-and time-dependent manner. Moreover, OFX could arrest the cells at S phase of the cell cycle and induce phosphatidylserine externalization, DNA fragmentation, apoptotic body formation, activations of caspase-2, -9, and-3, up-regulation of cytoplasmic cytochrome c and apoptosis inducing factor, and disruption of MTP. In conclusion, OFX above 1/8 of its clinical therapeutic concentration 山 东 大 学 学 报 (理 学 版)第51卷 - 第5期王德平,等:氧氟沙星对人角膜上皮细胞的毒性作用及其细胞与分子机理研究 \=-has a significant cytotoxicity to HCEP cells by inducing cell cycle arrest and apoptosis which is regulated by a death receptor-mediated mitochondrion-dependent signaling pathway.

Effects of simulated nitrogen deposition on soil microbial biomass and community structure in a young plantation of Castanopsis hystrix

HONG Pi-zheng, LIU Shi-rong, YU Hao-long, HAO Jian

JOURNAL OF SHANDONG UNIVERSITY(NATURAL SCIENCE). 2016, 51(5):  18-28.  doi:10.6040/j.issn.1671-9352.0.2015.214

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By using fumigation-extraction method and phospholipid fatty acid technique, the effects of simulated nitrogen deposition on soil microbial biomass and community structure in a young Castanopsis hystrix plantation of subtropical China were determined through a field N addition experiment. Nitrogen was added at rate of 0 kg/(hm²·year)(Control), 50 kg/(hm²·year)(Low-N), 100 kg/(hm²·year)(Medium-N)and 150 kg/(hm²·year)(High-N), respectively for 17 months. The main results were as follows: Medium-N and High-N treatments significantly increased soil N availability, but significantly decreased soil pH in the test plantation. In addition, all the N addition treatments did not significantly influenced the content of SOC, while Medium-N and High-N treatments significantly decreased the 山 东 大 学 学 报 (理 学 版)第51卷 - 第5期洪丕征,等:模拟氮沉降对红椎人工幼龄林土壤微生物生物量和微生物群落结构的影响 \=-ratio of soil C/N, but significantly increased the content of soil extractable DOC. Medium-N and High-N treatments significantly decreased the content of MBC, but significantly increased the content of MBN compared with the control plots. Moreover, Medium-N and High-N treatments significantly decreased soil total PLFAs, fungal PLFAs, and bacterial PLFAs. Besides, all the N addition treatments significantly decreased the relative abundance of gram-negative bacterial PLFAs, but significantly increased the relative abundance of actinomycetes PLFAs. However, only High-N treatment significantly decreased the relative abundance of gram-positive bacterial PLFAs. In addition, Medium-N and High-N addition treatments significantly increased the biomass ratio of fungal to bacterial. We also found that N addition significantly affected soil microbial community structure through PCA analysis of 27 individual PLFAs detected in the test soils. Moreover, the redundancy analysis(RDA)demonstrated that the shift of soil microbial community structure under simulated N deposition was significantly correlated to the variations in MBN and soil pH. These results suggest that nitrogen deposition could significantly influence the stability of soil microbial community and thus negatively affect soil health and fertility in the young plantation of C.hystrix.

Growth and physiological changes during plant senescence of ancient Pinus tabulaeformis Carr.

WANG Qiao, ZHU Hong, WANG Hua-tian, MENG Xian-Peng,CAO Gui-ping, LI Jian, HUANG Chang-bao

JOURNAL OF SHANDONG UNIVERSITY(NATURAL SCIENCE). 2016, 51(5):  29-35.  doi:10.6040/j.issn.1671-9352.0.2015.265

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In order to provide scientific basis of the vigor diagnosis and their rejuvenation and management to anti-aging, the growth and physiological diagnosis indices of the young healthy trees and senescence ancient pines were investigated. The annual pine needles from healthy and weak pines with different ages in the Taishan National Park were used as materials. The shoot length, needle length and coarseness, chlorophyll content, membrane permeability, net photosynthetic rate(P_n), transpiration rate(T_r), stomatal conductance(G_s), intercellular CO₂ concentration(C_i), mineral elements content of C, N, P, K and Mg, abscisic acid(ABA)content of pine needles were measured. The results showed that, comparing with healthy pines, the ancient ones had thinner, shorter needles and weaker photosynthetic capacity, lower growth of shoots, chlorophyll content and light use efficiency. Membrane permeability creased with membrane lipid peroxidation. There were no remarkable changes in P content and increased mineral elements content of C, 山 东 大 学 学 报 (理 学 版)第51卷 - 第5期王巧,等:油松古树衰老过程中的生长与生理变化 \=-N, Mg and K in senescing and weak ancient pines. Higher ABA content led to weakness of vigor. The correlation between morphological changes and physiological indexes were significant. It was summarized that growth and physiological indexes could be used as bases to diagnose the senescence and weakness of ancient pines.

Purification, characterization and gene cloning of the extracellular protease EYHⅠ from Salinivibrio sp.YH4

WU Cui-ling, LIU Dan, YANG Xing-hao, WU Ri-bang, HUANG Jia-feng, ZHANG Jiang, LUN Zi-feng, HE Hai-lun

JOURNAL OF SHANDONG UNIVERSITY(NATURAL SCIENCE). 2016, 51(5):  36-42.  doi:10.6040/j.issn.1671-9352.0.2015.609

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The extracellular proteases from moderate halophilic strain, Salinivibrio sp. YH4 was isolated, purified, identified and characterized to provide data for enzyme system of Salinivibrio sp.. Strain was cultured in liquid fermentation medium, the extracellular proteases was purified by using HiTrap Capto DEAE column chromatography and Gel filtration chromatography. The protease was identified by using mass spectrometry(MS)and designated as EYHⅠ. The metalloprotease EYHⅠ had optimal activity at 55 ℃ and pH 9.0, stabilized at 60 ℃ and pH 7.0~11.0. EYHⅠshowed higher proteolytic activity at 4 mol/L NaCl. The nucleotide sequence contains 1836 bp, encoding EYHⅠof 611 amino acid residues, which is highly conserved to zinc metalloprotease precursor in Salinivibrio sp. AF-2004 with 96% sequence similarity. Analysis of the structure showed that EYHⅠcontained an FTP domain,a PepSY domain, M4 neutral protease and a PPC domain. These results may provide important data for the study of Salinivibrio sp. and the application of protease EYHⅠ.

A preliminary study on cold resistance of four introduced oaks seedlings

YANG Zhen-ya, TAI Xiu-guo, CAO Zhen-yu, LI Bo, CAO Bang-hua, WANG Shu-mei, WANG Xuan

JOURNAL OF SHANDONG UNIVERSITY(NATURAL SCIENCE). 2016, 51(5):  43-47.  doi:10.6040/j.issn.1671-9352.0.2015.573

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Dormant branches of four oak trees(Quercus nuttallii, Quercus palustris, Quercus shumardii, Quercus bicolor)were used as materials to measure the changes of physiological and biochemical indexes(the electrolyte leakage,POD, SOD, soluble protein, MDA content)under different low temperature condition(0 ℃、-5 ℃、-10 ℃、-15 ℃、-20 ℃、-25 ℃、-30 ℃、-35 ℃ in this paper.Logistic equation was applied to fitting change rules of the electrolyte leakage, the results showed that the fitting effect is remarkable and correlation coefficient all are above 0.90. Semi-lethal temperature(LT50)and cold resistance rankings of four kinds of oak trees were obtained by parameters analyzing:palustris, Quercus (-28.42 ℃)>Quercus nuttallii(-25.11 ℃)>Quercus bicolor (-24.78 ℃)>Quercus shumardii (-22.42 ℃).

The investigation of the gravity darkening and evolution in rotating star

TAI Li-ting, SONG Han-feng, WANG Jiang-tao, ZHAN Qiong

JOURNAL OF SHANDONG UNIVERSITY(NATURAL SCIENCE). 2016, 51(5):  48-60.  doi:10.6040/j.issn.1671-9352.0.2015.164

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Rotation and tide are two important factors which have very important impact on the stellar structure and evolution. Based on the observational data of Achernar, we have investigated the gravitational darkening with the models including the effects of rotation and tide. We find that the rotating binaries can be more consistent with the observations of the ratio of equatorial radius to the polar one than the single rotating model. It is shown that the star with the initial larger angular velocity has bigger radius and central density at the beginning and middle of the main sequence, and it has smaller radius and central density due to the large loss of spin angular momentum in subsequent evolution. Also, we have found that rotation can make the star more centrally condensed and enlarged in size, decreasing the quadrupolar momentum, the gyration radius, central temperature, and the rate of the hydrogen production energy in the stellar core. The star shifts towards low temperature and luminosity in the HR diagram in rotating model.

A theory research and experiment scheme on enable-control Anderson localization and delocalization based on quantum walk

REN Chun-nian, GAO Jian

JOURNAL OF SHANDONG UNIVERSITY(NATURAL SCIENCE). 2016, 51(5):  61-66.  doi:10.6040/j.issn.1671-9352.0.2015.237

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A controllable scheme of the Anderson localization and delocalization that is based on the existing experiment of quantum walk is proposed. By the introduction of the adjustable parameters general coin and calculation results, the theory of one and two dimensional control experimental schemes are analyzed. Based on the theoretical results, an integrated optical waveguide and optical fiber devices with controllable Anderson effect experiment scheme is designed. The numerical simulation results show that the localization and delocalization is controlled by electronic polarizer and the rationality of the proposed scheme.

Attack on an image cipher by combining hyper chaotic sequences and shift operation

ZHU Cong-xu, LU Qing

JOURNAL OF SHANDONG UNIVERSITY(NATURAL SCIENCE). 2016, 51(5):  67-71.  doi:10.6040/j.issn.1671-9352.2.2015.310

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A chaos-based cryptosystem has exhibited some unconventionally excellent properties. At the same time, it is of vital significance for its advancement to give a mathematically rigorous cryptanalysis. In this study, we analyze the security of an image encryption algorithm, whose main idea is to shift the images pixel positions by using one of the improved hyperchaotic sequences, diffuse and confuse the image's pixel values by using shift cipher and other improved hyperchaotic sequences. By applying chosen plaintext attacks, we show that all the equivalent keys can be revealed, which can be exploited to reveal the plain image. Both mathematical analysis and experimental results are presented and the feasibility of the proposed chosen plaintext attack strategies is verified. Finally, some possible improved methods to increase the security of the image encryption algorithm are discussed briefly.

Design and implementation of transparent key transmission based on SMM

ZHANG Kun, ZHAO Bo, AN Yang

JOURNAL OF SHANDONG UNIVERSITY(NATURAL SCIENCE). 2016, 51(5):  72-77.  doi:10.6040/j.issn.1671-9352.2.2015.253

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The encryption key used in the driver layer encryption was usually stored in the peripheral equipment of USB. In the process of encryption, the key is transmitted to the driver layer via USB interface. But the USB channel is not secure enough and there may be key leakage threat. Therefore this paper proposed a secure key transmission solution based on system management module(SMM)to solve this problem. The solution leveraged the intangibility of SMM towards the operating system and we propose a new and secure key transmit scheme. The experiments results indicated that it could effectively resist the attack to USB channel, guarantee the key security during transmission and significantly enhance the security of encryption key in the driver layer.

Searchable encryption scheme based on biometrics

ZHU Bin-rui, QIN Jing, HAN Fei

JOURNAL OF SHANDONG UNIVERSITY(NATURAL SCIENCE). 2016, 51(5):  78-86.  doi:10.6040/j.issn.1671-9352.0.2015.269

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With the advent of the cloud era, more and more users would like to store their data to the cloud server, so it was convenient to use and reduce the storage cost. Since cloud server might not be enough safe and reliable, in order to keep the security, data were usually stored in encrypted form in the cloud. Searchable encryption focused on the safety, reliability, and efficiency of search process, ensured that the user can support keyword search over encrypted data, and did not affect its use experience at the same time. Compared with traditional passwords, biometrics passwords were more convenient to be taken and hard to be forgotten. This paper would construct a new searchable encryption system based on biometrics and proposed two schemes based on different difficult problems. Compared with the existing searchable encryption schemes, with combining biometrics, our new schemes improved the flexibility, practicability and security. In this paper, our scheme satisfied the safety of indistinguishability of chosen keywords and trapdoor.

Feature selection combined with the global and local information(GLFS)

WAN Zhong-ying, WANG Ming-wen, ZUO Jia-li, WAN Jian-yi

JOURNAL OF SHANDONG UNIVERSITY(NATURAL SCIENCE). 2016, 51(5):  87-93.  doi:10.6040/j.issn.1671-9352.1.2015.E17

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Feature selection methods directly affect the effect of text categorization. Traditional feature selection algorithm is based on global approach, ignoring the influence of local features, and even makes a lot of training document has no features. Therefore, the paper proposed a feature selection algorithm combined with the ALOFT method, which unify the traditional globe features and contribution rate of a word to individual document to unify the global and local information(GLFS). Experimental results in the Reuters data set and Fudan data set show that the method can ensure that each document has a characteristic word and improve classification performance. Furthermore, the paper discussed the influence of the new method of feature weights to classification.

Knowledge vein-based recommendation of academic papers

TAN Hong-ye, YAO Yi-lu, LIANG Ying-hong

JOURNAL OF SHANDONG UNIVERSITY(NATURAL SCIENCE). 2016, 51(5):  94-101.  doi:10.6040/j.issn.1671-9352.1.2015.E15

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This paper proposed a method of scientific papers recommendation based on knowledge vein, which identified the authors of paper with their keywords and build a knowledge vein by using the synonymy, hyponymy and co-occurrence relationship of keywords. This method recommended academic papers for authors combined with CBF and knowledge vein. This method definition that similarity between authors and papers is the average maximum semantic similarity of the keyword vectors. Currently this article considered two kinds of semantic relations, synonymous and hyponymy. In addition, the relation of co-occurrence also was considered. The results showed that this method can provide more accurate and credible scientific paper recommendation service for authors.

The fuzzy clustering algorithm based on inclusion index of Gausian membership function

ZHAI Peng, LI Deng-dao

JOURNAL OF SHANDONG UNIVERSITY(NATURAL SCIENCE). 2016, 51(5):  102-105.  doi:10.6040/j.issn.1671-9352.3.2015.105

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Based on traditional fuzzy clustering algorithm, such as Fuzzy algorithm and C-means algorithm, the definition of inclusion index is taken into account the fuzzy clustering algorithm proposed, which is based on Gaussian membership functions. This algorithm ensure that the distance of each classification to another classifications is the same by a semi definite matrix, which preserves the inclusion index of Gausian membership function. The simulation experiment results with the Wine data set of UCI show that, compared with FCM, FIC algorithm has more effectiveness and feasibility.

On construction of resilient functions with maximum algebraic immunity

SUN Tian-feng, HU Bin

JOURNAL OF SHANDONG UNIVERSITY(NATURAL SCIENCE). 2016, 51(5):  106-113.  doi:10.6040/j.issn.1671-9352.0.2015.295

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The construction of resilient functions with maximum algebraic immunity was studied by a new method of flexibility, which can be carried out by software implementation easily. By means of flexibility, any majority function can be transformed into resilient function. A sufficient and necessary condition on which the resilient function has maximum algebraic immunity was obtained. Moreover, the cryptographic properties of the resilient function, such as nonlinearity and algebraic degree were studied.

Lyapunov-type stabilizating conditions of discrete-time stochastic systems with input delay

TAN Cheng, ZHANG Huan-shui

JOURNAL OF SHANDONG UNIVERSITY(NATURAL SCIENCE). 2016, 51(5):  114-120.  doi:10.6040/j.issn.1671-9352.0.2015.507

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This paper mainly studies the asymptotical mean square stabilization problem for discrete-time stochastic system with single input delay and multiplicative noises. First, expressed by Lyapunov-type inequalities, some sufficient and easily verified stabilizing conditions in mean square sense are developed. Second, based on the derived coupled Lyapunov-type equations(CLEs), a necessary condition is developed. It is remarkable that when the considered stochastic system with input delay degrades into the stochastic system without input delay or the deterministic time-delay system, the reduced system is asymptotical mean square stabilizable if and only if the given CLEs have unique solutions.

The connection between DPP and MP for the fully coupled forward-backward stochastic control systems

NIE Tian-yang, SHI Jing-tao

JOURNAL OF SHANDONG UNIVERSITY(NATURAL SCIENCE). 2016, 51(5):  121-129.  doi:10.6040/j.issn.1671-9352.0.2015.383

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This paper is concerned with the connection between dynamic programming principle(DPP)and maximum principle(MP)for the forward-backward stochastic control system, where the recursive cost functional is defined as one of the solution to a controlled fully coupled forward-backward stochastic differential equation(FBSDE). With some smooth assumptions, relations among the value function, generalized Hamiltonian function and adjoint processes are given, when the diffusion coefficient of the forward equation does not contain the state variable z. The general case for the problem is open. A linear example is discussed as the illustration of our main result.

Mean square exponential stability of memristor-based stochastic neural networks with S-type distributed delays

WANG Chang-hong, WANG Lin-shan

JOURNAL OF SHANDONG UNIVERSITY(NATURAL SCIENCE). 2016, 51(5):  130-135.  doi:10.6040/j.issn.1671-9352.0.2015.545

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The mean square exponential stability of a class of memristor-based stochastic neural networks with S-type distributed delays is investigated. A criterion for the stability is given by using Lyapunov functional method and stochastic analysis technique. An example is given to show the feasibility of the theoretical results.

Optimal control for a nonlinear diffusion system with age-dependent

LIU Jiang-bi, LUO Zhi-xue

JOURNAL OF SHANDONG UNIVERSITY(NATURAL SCIENCE). 2016, 51(5):  136-142.  doi:10.6040/j.issn.1671-9352.0.2016.017

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In this paper, we investigate the optimal harvesting for a class of nonlinear diffusion problem in an age-dependent population system with fertility and mortality depending on the population size. Making the maximum revenue from harvested by control the proportion of the species harvested. Mazurs theory is used to obtain existence of an optimal control. Optimality conditions are derived by means of normal cone technique. Some results in references are extended.