JOURNAL OF SHANDONG UNIVERSITY(NATURAL SCIENCE) ›› 2017, Vol. 52 ›› Issue (9): 103-110.doi: 10.6040/j.issn.1671-9352.0.2017.155

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Heterelogous expression, purification andcrystal growth of gas vesicle protein GvpW from the cyanobacterial Microcystis aeruginosa

XU Bo-ying1,2, ZHANG Tian-tian1   

  1. 1. College of Life Sciences, Chongqing Normal University, Chongqing 401331, China;
    2. School of Life Sciences, University of Science and Technology of China, Heifei 230026, Anhui, China
  • Received:2017-04-12 Online:2017-09-20 Published:2017-09-15

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Abstract: Microcystis aeruginosa PCC 7806 is the most dominant algae of seasonal outbreak cyanobacteria bloom in fresh water lake ecosystem of China. One of the outbreak mechanisms for the occurrence of cyanobacterial water-bloom is gas vesicles, which are gas-filled proteinaceous organelles and provide varying buoyancy for cyanobacteria to regulate the position for growth and subsequent colonization. The gas vesicle of M. aeruginosa is composed of 14 gas vesicles proteins, but it is still unclear what the specific molecular functions of most Gvp proteins are during the process of gas vesicle synthesis. In this study, a gvpW gene was cloned and heterologous expressed in Escherichia coli, and the recombinant protein was purified by Ni2+ affinity chromatography and gel filtration. Dynamic light scattering and chemical crosslinking analyses showed that the recombinant protein GvpW mainly existed as monomer in vitro. In addition, GvpW protein crystals were obtained by crystal screening and optimization. These results provide a basis for further studies on its three-dimensional structure determination and molecular functions.

Key words: Microcystis aeruginosa, Gvp proteins, GvpW, protein crystals, gas vesicles

CLC Number: 

  • Q816
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