JOURNAL OF SHANDONG UNIVERSITY(NATURAL SCIENCE) ›› 2021, Vol. 56 ›› Issue (9): 21-27,49.doi: 10.6040/j.issn.1671-9352.0.2020.621

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Comparison analysis of microbial community structure in different functional areas of recirculating aquaculture system

Zhan-sheng GUO(),Yi DING,Hai-tao ZHANG,Xu-guang HOU*()   

  1. Marine College, Shandong University, Weihai 264209, Shandong, China
  • Received:2020-11-04 Online:2021-09-20 Published:2021-09-13
  • Contact: Xu-guang HOU E-mail:guozhansheng@sdu.edu.cn;houxuguang@sdu.edu.cn

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Abstract:

In order to explore the microbial community structure in different functional areas of recirculating aquaculture system, as well as to understand the relationship between environmental factors and the microbial community, microbial community structure in recirculating aquaculture system was analyzed by using high-throughput sequencing with 16S rRNA in this study. The results showed that Proteobacteria and Bacteroidetes were the most dominant phyla in overall system, the compositions of dominant genera were quite different in different areas of system, while the relative abundances of Vibrio in whole system were both exceeding 1%. α-diversity (Shannon, ACE and chao1) of samples collected from biofilter and Rapana venosa pond were highest, and that in abalone pond and natural seawater were lowest. Non-metric multi-dimensional scaling (NMDS) analysis showed the system areas possessed distinctive distances of microbial communities except samples between biofilter and culture pond inlet. The removal rates of ammonia nitrogen and nitrite nitrogen from biofilter to the inlet of culture pond were 8.77% and 45.12%, respectively. The concentrations of nitrate nitrogen maintained a high level in the whole system (148.50~200.47 mg/L). There was no significant correlations between environmental factors and microbial community structure, temperature and nitrite nitrogen had relatively stronger effects on microbial community structure. This study provides a reference for the design improvement and daily management of recirculating aquaculture system in the next step.

Key words: recirculating aquaculture system, functional area, high-throughput sequencing, microbial community

CLC Number: 

  • S969.39

Fig.1

Diagram of recirculating aquaculture system"

Table 1

Alpha diversity estimates of microbial community"

样品 有效序列 有效OTU Shannon指数 Chao1指数 ACE指数 测序覆盖率
BI 80149 1089 6.837 1041.619 1057.583 0.997
WI 80167 1029 5.746 1012.321 1021.027 0.997
HWO 80171 515 4.694 491.500 500.137 0.998
RWO 80078 1104 7.144 1197.774 1193.839 0.996
SWO 80087 906 6.771 905.210 898.597 0.997
MF 80005 992 7.079 989.000 996.671 0.997
NW 80130 436 4.533 434.553 427.336 0.998

Fig.2

Composition of microbial community structure in recirculating aquaculture system at phyla (a) and genera (b) levels"

Fig.3

Microbial community structure in recirculating aquaculture system as indicated by NMDS"

Table 2

Environmental factors in in different functional areas of recirculating aquaculture system"

采样区域 T/℃ pH ρ(DO)/(mg·L-1) ρ(NH4+)/(mg·L-1) ρ(NO2-)/(mg·L-1) ρ(NO3-)/(mg·L-1)
BI 15.1 8.42 6.92 0.0399 0.082 200.34
WI 15.6 8.43 7.33 0.0364 0.045 151.91
HWO 15.5 8.42 7.22 0.0415 0.083 148.50
RWO 15.4 8.42 7.20 0.0469 0.110 189.94
SWO 15.4 8.43 7.21 0.0371 0.075 156.86
MF 15.5 8.39 7.14 0.0383 0.093 200.47
NW 9.3 8.40 8.04 0.0363 0.032 33.84

Fig.4

CCA ordination plots of relationship between environmental variables and microbial community"

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