Abstract:

Sectional moving window factor analysis (SMWFA)heuristic evolving latent projection (HELP), are used to resolve the overlapped two-dimensional chromatogram-spectrum data of the Traditional Chinese Medicine into estimated pure component chromatograms and spectrum profiles. In this work, the method was used to resolve a heavily overlapped components cluster of the compound Haqing injection. On the basis of results of the method, an improved deducted order of magnitude of the fixed size moving window evolving factor analysis (DOMFSMWFA) procedure was proposed and applied in the resolution of the compound Haqing injection, and the satisfactory results were obtained.

Key words: chemometric； resolution; traditional Chinese medicine(TCM); heuristic evolving latent projection(HELP); compound Haqing injection; chromatography