J4 ›› 2010, Vol. 45 ›› Issue (1): 41-45.

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Determination of efficacious components of Gentianaceae plants and their calli by RP-HPLC

  

  1.  ZHAO Zhong-Juan, WANG Yan-Fei,  JIA Guang-Min, XIANG Feng-Ning:The Key Laboratory of Plant Cell Engineering and Germplasm Innovation, Ministry of Education,Schoold of Life Science, Shandong Univeristy, Jinan 250100, Shangdong, China CAI Yun-Fei: Department of Bioscience and Biotechnology, Shandong Institute of Education,Jinan 250013, Shandong, China
  • Received:2009-07-06 Online:2010-01-16 Published:2010-03-25

Abstract:

A developed reverse phase high performance liquid chromatography (RPHPLC) was used to determine the contents of five efficacious components (Swertiamarin, Gentiopicroside, Mangiferin, Oleanolic acid and Urolic acid) in Chinese medical herbs Swertia musstii Franch, Gentiana siphonantha Maxim, and Gentianella albiflora Maxim., Gentianopsis paludosa Ma. and their calli from subculturals. A Phenomenex Luna C18 column was used and the column temperature was room temperature. Swertiamarin, Gentiopicroside and Mangiferin were detected at 259nm with Vmethanol∶Vwater∶Vacetic acid=25.00∶75.00∶0.04 as mobile phase and 0.8?mL/min as flowrate. While by Vmethanol∶Vwater∶Vacetic acid=85.00∶15.00∶0.04 as mobile phase, 1.0mL/min as flow rate and 209?nm as wavelength, and the contents of Oleanolic and Urolic acid were analyzed. The kind and content of efficacious components from the four Chinese medical herbs were quite different. Swertia -musstii-Franch contained the most efficacious components and its contents were the highest. The contents of five efficacious compounds in the above four medical herbs calli had less efficacious compounds than that of wild plants and their changes were different. However, according to the projected growth rate, the content of efficacious components accumulation of the callus was much higher than that of the wild plant. This is a precise and quick method to analyze the efficacious components inChinese medical herbs, which provides the basic for large-scale production of efficacious components in Chinese medical herbs.

Key words:  reversed-phase high performance liquid chromatography (RP-HPLC); chinese medical herbs; calli; efficacious components

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