J4 ›› 2010, Vol. 45 ›› Issue (5): 19-21.

• Articles • Previous Articles     Next Articles

Determination of melamine in eggs by high performance liquid chromatography

WEI Jin-mei, ZHANG Yong-mei, CAO Lu-xing, LUO Yu-zhu*   

  1. Instrumental Research and Analysis Center, Gansu Agricultural University, Lanzhou 730070, Gansu, China
  • Received:2009-08-20 Online:2010-05-16 Published:2010-05-24


A method was reported for the determination of melamine in eggs. After being extracted by 1%Trichloroacetic acid solution and 2.2% lead acetate from samples,melamine was separated on a Hypersil ODS 100×4.6mm,5μm C18 column with a volume ratio of acetonitrile and sulfonic sodium octane(pH=3) of 15∶85,  and  was detected by an ultraviolet detector at a wavelength of 210nm.The temperature of column was 40℃. The linear ranges were from 0.2μg/mL to 10.0μg/mL. The results indicated that the average recoveries were 82.0%~104.0% for high, middle and low concentration levels by this method. The relative standard deviations RSD was 6.81%(n=9).

Key words: melamine; eggs; high performance liquid chromatography(HPLC)

No related articles found!
Full text



No Suggested Reading articles found!