J4 ›› 2010, Vol. 45 ›› Issue (5): 19-21.
• Articles •
WEI Jin-mei, ZHANG Yong-mei, CAO Lu-xing, LUO Yu-zhu*
A method was reported for the determination of melamine in eggs. After being extracted by 1％Trichloroacetic acid solution and 2.2％ lead acetate from samples,melamine was separated on a Hypersil ODS 100×4.6mm,5μm C18 column with a volume ratio of acetonitrile and sulfonic sodium octane(pH=3) of 15∶85, and was detected by an ultraviolet detector at a wavelength of 210nm.The temperature of column was 40℃. The linear ranges were from 0.2μg／mL to 10.0μg／mL. The results indicated that the average recoveries were 82.0％~104.0％ for high, middle and low concentration levels by this method. The relative standard deviations RSD was 6.81％(n=9).
melamine; eggs; high performance liquid chromatography(HPLC)
WEI Jin-mei, ZHANG Yong-mei, CAO Lu-xing, LUO Yu-zhu*. Determination of melamine in eggs by high performance liquid chromatography[J].J4, 2010, 45(5): 19-21.
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