J4 ›› 2011, Vol. 46 ›› Issue (11): 12-16.

• Articles • Previous Articles     Next Articles

A highly sensitive chemiluminescent enzymelinked immunosorbent assay for the determination of lomefloxacin

CAO Zhao-zhen1, LU Sheng-xin1, XI Ri-mo2*   

  1. 1. School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, Shandong, China;
    2. College of Pharmacy, Nankai University, Tianjin 300071, China
  • Received:2011-05-23 Online:2011-11-20 Published:2011-11-30


A chemiluminescent enzyme-linked immumosorbent assay (CL-ELISA) based on polyclonal antibody for lomefloxacin was developed. Assay conditions, including the concentration of coating antigen, the dilution degree of the primary antibody and IgG-HRP, and the type of micro-titer plate. were studied. The optimized CL-ELISA presented high sensitivity with an IC50 value of 0.22ng/mL and detection limit of 0.032ng/mL. Good specificity was acquired with only norfloxacin and fleroxacin showing a percentage of cross-reactivity higher than 1% (6.88% and 2.16% respectively) among the ten detected representative quinolones. The method developed was applied in the detection of lomefloxacin recovery in spiked milk. The average recovery rates were in the ranges of 88.7%~103.4% and 97.8%~107.2% for intra- and inter-assay respectively while the corresponding coefficient of variation (CV%) were in the ranges of  6.3%~ 8.6% and 2.4%~ 8.0%.

Key words: antibody; chemiluminesence; ELISA; drug residue; lomefloxacin

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