Abstract:

 To test the performance of new bolaamphiphile used as a gene carrier, compounds of the bola and siRNA-bolaplexes were prepared. The morphology of bolaplexes at different weight ratios of bola and siRNA was observed with AFM (Atomic force microscope). After Hela cells were transfected, the cytotoxicity of bolaplexes was detected with the CCK-8 kit, and the swallow effect was inspected with FM (fluorescence microscope) and FC (flow cyctometry). The result of AFM showed that at the condition of weight ratio of 1∶1, the size of the bolaplexes was about 100-200nm, and they could access Hela cells through endocytosis with high efficiency. Toxic experimental results showed that the cytotoxicity of bolaplexes was inferior to that of the compounds of Lipofectamine2000TM and siRNA, so bolaplexes have a good prospect for applications. This study laid a foundation for the clinical study of bolaamphiphile used for gene therapy.

Key words:  bolaamphiphile; gene therapy; nonviral vector; RNA interference