Abstract:

The degradation of aromatic compounds has been widely studied biochemically or genetically. Through 2-Dimensional gel electrophoresis (2-DGE) and Matrix assisted laser desorption／ionization-Time of flight (MALDI-TOF) mass spectrometry analysis, the expression of mono cyclic aromatic compounds degradation related proteins were compared when bacteria Pseudomonas putida F1 was grown on succinate, touene, ethylbenzene or the mixture of toluene and ethylbenzene as carbon and energy sources. Although the aromatic compounds were all metabolized using TOD pathway, the expression of some aromatic hydrocarbon degradation related proteins were different when the cells were grown on different carbon sources. Four groups of differentially expressed proteins have been identified, including metabolic proteins, transportation proteins, adaptation related proteins and other proteins.

Key words: proteomics; 2-DGE; MALDI-TOF; monocyclic aromatic hydrocarbon; protein expression