JOURNAL OF SHANDONG UNIVERSITY(NATURAL SCIENCE) ›› 2020, Vol. 55 ›› Issue (9): 102-110.doi: 10.6040/j.issn.1671-9352.0.2019.834

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Pathological study on infection of Vibrio anguillarum and Vibrio parahemolyticus to Octopus ocellatus

Yan-wei FENG2,Zan LI2,Wei-jun WANG2,Guo-hua SUN2,Guo-hua SUN2,Xiao-hui XU2,*()   

  1. 1. College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China
    2. Agricultural College, Ludong University, Yantai 264025, Shandong, China
    3. Shanghai Aquaculture Engineering Technology Research Center, Shanghai Ocean University, Shanghai 201306, China
  • Received:2019-11-25 Online:2020-09-20 Published:2020-09-17
  • Contact: Xiao-hui XU E-mail:up@126.com

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Abstract:

In order to control aquaculture diseases and understand the influence of pathogen microorganisms on cephalopoda culture, we selected vibrio with high incidence in seawater culture, including V.anguillarum and V.parahemolyticus, to study their virulence level and pathogenic mechanism against shortarm octopus. Through direct injection of diluted bacteria solution into shortarm octopus carpus arm, the death rate and half lethal time of shortarm octopus after infection were observed, as well as the effects of various bacteria on the structures of shortarm octopus liver, gill, gill heart and white body. The results showed that V.anguillarum and V.parahemolyticus were both highly pathogenic to shortarm octopus, reaching a semi-fatality rate at 60 h and 48 h, respectively. In addition, the activity of octopus was decreased, weakness and abdominal swelling were observed; Through the observation of paraffin sections and HE staining and ultramicroscopic structure of diseased and healthy tissues, it was found that the liver, gill, gill heart and white body of the diseased octopus showed different degrees of tissue or cell structure damage. The above results indicated that both V.anguillarum and V.parahemolyticus were significantly pathogenic to shortarm octopus, and the pathogenicity of V.parahaemolyticus might be stronger, which would be one of the greatest threats of shortarm octopus large-scale farming if the conditions were suitable.

Key words: Octopus ocellatus, Vibrio anguillarum, Vibrio Parahemolyticus, infection

CLC Number: 

  • Q17

Table 1

Variation of survival number of O.octopus after vibrio infection in each time period"

组别时间/h
0 12 24 36 48 60 72 96 120
对照组(C)/只 16 16 16 15 15 15 15 15 15
鳗弧菌感染组(A)/只 16 16 15 14 11 8 6 2 0
副溶血弧菌感染组(B)/只 16 16 13 10 8 6 3 1 0

Fig.1

Paraffin sections and HE staining of O.octopus s liver and gill tissues(A and B represent liver and gill tissue repectively, 0—2 repectively represent the control group, V.anguillae infection group and V.parehaemolyticus infection group)"

Fig.2

Paraffin sections and HE staining of O.octopus s gill heart and white body tissues(C and D represent gill heart and white body repectively, 0—2 repectively represent the control group, V.anguillae infection group and V.parehaemolyticus infection group)"

Fig.3

Transmission electron microscope analysis of O.octopu s liver and gill tissues of infection(E and F represent liver and gill tissus respectively, 0-2 repectively represent the control group, V.anguillae infection group and V.parehaemolyticus infection group, N stands for nucleus, CP stands for cytoplasm, the black arrow points to the secretory cells in the gills, the white arrow points to the epithelial microvilli in the gills)"

Fig.4

Transmission electron microscope analysis of O.octopus gill heart and white body tissues of infection(E and F represent goll heart and white body tissus respectively, 0—2 repectively represent the control group, V.anguillae infection group and V.parehaemolyticus infection group, N stands for nucleus, CP stands for cytoplasm, DB stands for electron-dense body)"

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