JOURNAL OF SHANDONG UNIVERSITY(NATURAL SCIENCE) ›› 2015, Vol. 50 ›› Issue (11): 1-7.doi: 10.6040/j.issn.1671-9352.0.2014.376

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Chromokinesin KIF4A inhibits migration of gastric cancer cells

HU Xiao-qing1,3, LIANG Shuang-shuang1,3, JI Mei-chao1,3, ZHU Chang-jun1,2,3, PAN Li-na1,2,3   

  1. 1. College of Life Science, Tianjin Normal University, Tianjin 300387, China;
    2. Tianjin Key Laboratory of Animal and Plant Resistance, Tianjin 300387, China;
    3. Key Laboratory of Molecular and Cellular Systems Biology, Tianjin Normal University, Tianjin 300387, China
  • Received:2014-08-20 Revised:2015-01-15 Online:2015-11-20 Published:2015-12-09

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Abstract: To explore the role of chromokinesin KIF4A regulating migration of gastric cancer cells and then provide a potential therapeutic targets for clinical treatment of gastric cancer. Gastric cancer cell lines were constructed with either stably lower-expression or stably over-expression of KIF4A using G418 selection. Then we verified these cell lines using Western Bolt and immunofluorescent staining. These cells' migration abilities were assayed with wound healing and Transwell experiments. KIF4A stably lower-expression (SGC-shKIF4A) or overexpression cell lines (SGC-KIF4A) were successfully constructed and cell migration of SGC-shKIF4A cells were faster than that of SGC-KIF4A cell lines. Chromokinesin KIF4A inhibits the ability of gastric cancer cell movement laying a foundation for studies of KIF4A's roles in gastric cancer metastasis.

Key words: chromokinesin, Gastric cancer, cell migration, KIF4A

CLC Number: 

  • Q78
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