基于宏基因组测序技术分析连作对杨树人工林土壤微生物群落的影响

doi:10.6040/j.issn.1671-9352.3.2018.385

Abstract

Abstract:

The metagenome sequencing was used to analyze the changes of microbial community between the first and the second continuous cropping of poplar plantations for understanding the effect of successive-planting on soil microbial community, meanwhile the content of phenolic acid in soil was analyzed. The results showed that there were no significant differences in soil microbial community diversity and uniformity while significant differences (P < 0.05) were found in the composition and abundance of microbial community after successive-planting of poplar plantation. Microorganisms from eight categories and sixty-three genera were found with Bacillus being the dominant. The continuous cropping had significant effects on soil microbial community composition and abundance in poplar plantations. The relative abundance of nine genera microbes was significantly increased(P < 0.05) and the relative abundance of seven genera was significantly decreased (P < 0.05) in the soil of second generation forest. The relative abundance of genes related to metabolism such as DNA repair and protein recombination, secondary metabolites biosynthesis, transport and catabolism was decreased. The total content of phenolic acids in soil was significantly increased (P < 0.05), and there was a general correlation between the variation of microbial diversity and phenolic acid content.

Key words: poplar plantation, successive-planting, metagenome sequencing, microbes, phenolic acid

CLC Number:

S718.8

Ying ZHANG,Xue-song MA,Ru-yan JING,Feng-yun MA,Jian-yao GUO,Yan-ping WANG,Hua-tian WANG. Effects of successive-planting poplar plantation on soil microbial community[J].JOURNAL OF SHANDONG UNIVERSITY(NATURAL SCIENCE), 2019, 54(1): 36-46.

Figures/Tables 10

Table 1

Fig.1

Fig.2

Table 2

Changes in relative abundance of the microorganisms at the phyla and genera level of poplar plantation"

分类			相对丰度
门	属		Ⅰ代林P1	Ⅱ代林P2
厚壁菌门Firmicutes			62.42±1.22a	63.28±1.25a
	芽孢杆菌属Bacillus		62.09±1.22a	62.78±1.09a
	杆菌属Lysinibacillus		0.01±0.00a	0.00±0.00b
	类芽孢杆菌属Paenibacillus		0.00±0.00b	0.01±0.01a
	明串球菌属Leuconostoc		0.03±0.01a	0.04±0.03a
	链球菌属Streptococcus		0.24±0.03a	0.36±0.13a
	乳球菌属Lactococcus		0.06±0.02a	0.09±0.01a
变形菌门Proteobacteria			19.69±0.72a	19.31±0.41a
	假单胞菌属Pseudomonas		5.37±0.07b	5.58±0.05a
	不动杆菌属Acinetobacter		0.48±0.16a	0.41±0.12a
	水栖菌属Enhydrobacter		0.66±0.10a	0.73±0.10a
	Thioalkalivibrio		0.47±0.18a	0.22±0.07a
	柄杆菌属Caulobacter		0.06±0.04a	0.04±0.01a
	军团菌属Legionella		0.08±0.01a	0.04±0.01b
	寡养单胞菌属Stenotrophomonas		0.07±0.01a	0.04±0.01a
	亚硝化螺菌属Nitrosospira		0.02±0.01a	0.04±0.01a
	堆囊菌属Sorangium		0.01±0.00a	0.04±0.02a
	Desulfobulbaceae_unclassified		0.00±0.00b	0.02±0.01a
	泛生菌属Pantoea		0.01±0.01a	0.00±0.00b
	苯基杆菌属Phenylobacterium		0.04±0.01a	0.01±0.01a
	Aurantimonadaceae_unclassified		0.19±0.06a	0.10±0.01a
	Beijerinckiaceae_unclassified		0.36±0.01a	0.36±0.05a
	慢生根瘤菌属Bradyrhizobium		0.68±0.10b	1.06±0.03a
	阿菲波菌属Afipia		0.05±0.01a	0.05±0.03a
	红假单胞菌属Rhodopseudomonas		1.10±0.20b	2.01±0.10a
	硝化菌属Nitrobacter		0.13±0.02a	0.11±0.02a
	甲基杆菌属Methylobacterium		0.57±0.11a	0.69±0.21a
	Methylocystaceae_unclassified		1.85±0.39a	1.75±0.54a
	中慢生根瘤菌属Mesorhizobium		0.26±0.08a	0.31±0.16a
	根瘤菌属Rhizobium		0.05±0.02b	0.12±0.02a
	Xanthobacteraceae_unclassified		0.09±0.02a	0.08±0.05a
	Acetobacteraceae_unclassified		1.01±0.01a	0.67±0.04b
	Erythrobacteraceae_unclassified		0.02±0.01a	0.00±0.00b
	波氏杆菌属Bordetella		0.06±0.01a	0.04±0.01b
	鞘脂单胞菌属Sphingopyxis		0.04±0.02a	0.00±0.00b
	伯克氏菌属Burkholderia		5.54±0.79a	4.61±0.24a
	贪噬菌属Variovorax		0.16±0.06a	0.16±0.05a
	罗尔斯通菌属Ralstonia		0.04±0.01a	0.02±0.01a
	贪铜菌属Cupriavidus		0.24±0.06a	0.00±0.00b
放线菌门Actinobacteria			13.62±0.27a	13.07±0.52a
	Micromonosporaceae_unclassified		3.96±0.63a	3.22±0.21b
	Catenulispora		0.27±0.05a	0.50±0.03a
	弗兰克氏菌属Frankia		0.23±0.04b	0.47±0.04a
	地嗜皮菌属Geodermatophilus		0.05±0.02a	0.04±0.02a
	赖氏菌属Leifsonia		0.02±0.01a	0.01±0.01a
	小单孢菌属Micromonospora		0.07±0.02a	0.01±0.01b
	节细菌属Arthrobacter		1.06±0.06b	1.69±0.08a
	盐孢菌属Salinispora		0.002±0.001a	0.00±0.000b
	分歧杆菌属Mycobacterium		7.07±0.07a	5.70±0.14b
	Nakamurella		0.00±0.00b	0.02±0.01a
	Kribbella		0.03±0.01a	0.04±0.01a
	类诺卡氏菌属Nocardioides		0.004±0.001a	0.00±0.000b
	丙酸杆菌属Propionibacterium		0.01±0.01a	0.00±0.00b
	拟无枝酸菌属Amycolatopsis		0.27±0.11b	0.72±0.16a
	链霉菌属Streptomyces		0.41±0.10a	0.58±0.13a
	链孢子囊菌属Streptosporangium		0.09±0.04a	0.00±0.00b
	双歧杆菌属Bifidobacterium		0.01±0.01a	0.00±0.00b
	Conexibacter		0.06±0.04a	0.08±0.04a
酸杆菌门Acidobacteri			1.88±0.53a	1.31±0.44a
	Granulicella		1.88±0.53a	1.31±0.44a
	Korebacter		0.01±0.01a	0.00±0.00b
拟杆菌门Bacteroidetes			1.49±0.41a	2.01±0.20a
	Sphingobacteriaceae_unclassified		1.43±0.42a	1.84±0.19a
	黄杆菌属Flavobacterium		0.07±0.02b	0.17±0.04a
绿弯菌门Chloroflexi		0.51±0.02b	0.84±0.01a
	纤线杆菌属Ktedonobacter	0.06±0.02b	0.16±0.01a
疣微菌门Verrucomicrobia		0.37±0.05a	0.19±0.03b
	Chthoniobacter	0.36±0.05a	0.19±0.03b
	丰佑菌属Opitutus	0.01±0.00a	0.00±0.00b
浮霉菌门Planctomycetes		0.01±0.01a	0.01±0.01a
	出芽菌属Gemmata		0.01±0.01a	0.01±0.01a

Table 2

Fig.3

Fig.4

Fig.5

Table 3

Table 4

Table 5

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土样	pH	有机质含量/(mg·g^-1)	全磷含量/(mg·g^-1)	有效磷含量/(μg·g^-1)	有效钾含量/(μg·g^-1)	有效氮含量/(mg·g^-1)
P1	6.17±0.01b	12.18±0.78b	0.10±0.15c	25.56±1.42a	78.36±2.07b	40.65±1.04b
P2	6.12±0.01c	15.42±1.38a	0.14±0.01ab	22.84±1.19a	75.89±4.42b	40.14±2.71b

土样	编码蛋白数(10⁶)	KEGG同源基因数	eggNOG同源基因数
P1	4.70±0.16a	6 534±112a	43 580±219a
P2	4.58±0.16ab	6 520±58a	43 182±576a

土样	对羟基苯甲酸	香草醛	阿魏酸	苯甲酸	肉桂酸	总量
P1	13.31±0.03b	3.16±0.01a	5.89±0.33a	6.73±0.15b	0.53±0.02b	29.63±0.23b
P2	15.93±0.14a	2.12±0.09b	5.11±0.18b	7.15±0.15a	0.81±0.07a	31.12±0.03a

属	对羟基苯甲酸	香草醛	阿魏酸	苯甲酸	肉桂酸	酚酸总量
假单胞菌属Pseudomonas	0.92^*	-0.92^**	-0.82^*	0.78	0.87^*	0.87^*
红假单胞菌属Rhodopseudomonas	0.97^**	-0.96^**	-0.74	0.75	0.94^**	0.99^**
节细菌属Arthrobacter	0.98^**	-0.97^**	-0.87^*	0.88^*	0.97^**	0.97^**
慢生根瘤菌属Bradyrhizobium	0.96^**	-0.95^**	-0.76	0.77	0.94^**	0.98^**
拟无枝酸菌属Amycolatopsis	0.91^*	-0.91^*	-0.64	0.63	0.83^*	0.93^**
弗兰克氏菌属Frankia	0.96^**	-0.97^**	-0.91^**	0.88^*	0.90^*	0.92^**
根瘤菌属Rhizobium	0.92^*	-0.91^*	-0.76	0.78	0.92^**	0.94^**
黄杆菌属Flavobacterium	0.87^*	-0.87^*	-0.82^*	0.82^*	0.88^*	0.86^*
纤线杆菌属Ktedonobacter	0.97^**	-0.98^**	-0.89^*	0.86^*	0.88^*	0.93^**
分歧杆菌属Mycobacterium	-0.99^**	0.99^**	0.86^*	-0.84^*	-0.93^**	-0.97^**
Micromonosporaceae_unclassified	-0.94^**	0.94^**	0.80	-0.76	-0.82^*	-0.97^**
Acetobacteraceae_unclassified	-0.99^**	0.99^**	0.85^*	-0.83^*	-0.93^**	-0.98^**
Chthoniobacter	-0.93^**	0.92^**	0.75	-0.76	-0.92^**	-0.95^**
小单孢菌属Micromonospora	-0.87^*	0.87^*	0.99^**	-0.97^**	-0.86^*	-0.80
军团菌属Legionella	-0.89^*	0.88^*	0.86^*	-0.88^*	-0.94^**	-0.89^*
波氏杆菌属Bordetella	-0.96^**	0.95^**	0.95^**	-0.94^**	-0.94^**	-0.92^*

Effects of successive-planting poplar plantation on soil microbial community

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