JOURNAL OF SHANDONG UNIVERSITY(NATURAL SCIENCE) ›› 2022, Vol. 57 ›› Issue (1): 1-7.doi: 10.6040/j.issn.1671-9352.0.2021.323

   

Comparision of the intestinal microflora diversity of four species of abalone

GUO Zhan-sheng, ZHANG Hai-tao, HOU Xu-guang*   

  1. Marine College, Shandong University, Weihai 264209, Shandong, China
  • Published:2021-12-21

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Abstract: The composition and diversity of intestinal microflora of Haliotis discus hannai, H. iris, H. rubra and the hybrid abalone(H. discus hannai ♀×H. iris ♂)were investigated using 16S rRNA high-throughput sequencing technology. The results showed that the OTU number, the values of Shannon index and Chao1 index were the highest in H. iris, and the lowest in H. rubra. The dominant phyla in the intestines of the four abalone species were Proteobacteria, Fusobacteria and Tenericutes, accounting for 61.93% to 95.78%. Among them, the absolute dominant phylum in H. discus hannai, H. iris and hybrid abalone was Proteobacteria, while that in H. rubra was Tenericutes. Psychrilyobacter, Vibrio and Mycoplasma were the predominant genera of the intestinal tract of the four species of abalone, accounting for 39.70% to 53.65%. 175 OTUs belong to the core microflora were detected in four species of abalone, a total of 11 OTUs have been found that the relative abundance exceeded 1%. Both Anosim and Adonis analyses found that the intestinal microflora differed significantly between hybrid abalone and H. rubra, hybrid abalone and H. iris, and H. discus hannai and H. rubra(P<0.05); LefSe was used to analyze the differences of microbial taxon composition among abalones and seven biomarkers were identified, including six biomarkers in H. iris and one in hybrid abalone.

Key words: abalone, high-throughput sequencing, intestinal microflora, core microflora

CLC Number: 

  • S938.8
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