JOURNAL OF SHANDONG UNIVERSITY(NATURAL SCIENCE) ›› 2024, Vol. 59 ›› Issue (11): 85-92.doi: 10.6040/j.issn.1671-9352.0.2024.316

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Enzymatic catalysis for methyl esterification of high acid value rice bran oil deodorized distillate

ZHOU You, LI Pandeng, HE Leiyu, SUN Lihui*   

  1. School of Chemical Engineering, Ocean and Life Sciences, Dalian University of Technology, Panjin 124221, Liaoning, China
  • Published:2024-11-29

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Abstract: In order to improve the utilization rate of rice bran oil deodorized distillate, methyl esterification of rice bran oil deodorized distillate was catalyzed by the liquid lipase Eversa Transform 2.0. The esterification rate and the content of free fatty acids in the system were used as the evaluation indicators. The effects of reaction time, enzyme amount, molar ratio of alcohol to oil, and water content on the esterification rate were studied by single factor experiments. On this basis, the response surface experiment was used to optimize the esterification conditions. The results showed that the optimal conditions for methyl esterification of rice bran oil deodorized distillate were 1.2% enzyme addition, 5.9∶1 molar ratio of alcohol to oil, 11.8% water content, and 9.6 h reaction time. Under these conditions, the esterification rate was 97.37%. In summary, the low-cost liquid lipase Eversa Transform 2.0 catalyzed the esterification of rice bran oil deodorized distillate, which could obtain a high esterification rate and provide a green and efficient pretreatment method for the separation and extraction of phytosterols, vitamin E, and other active components in the system.

Key words: rice bran oil deodorized distillate, high acid value oil, enzyme catalysis, response surface methodology, methyl esterification

CLC Number: 

  • Q503
[1] 张欢,贾怡文,陈小威,等. 稻米油脱臭馏出物中角鲨烯分离与纯化工艺的研究[J]. 河南工业大学学报(自然科学版), 2022, 43(6):30-36. ZHANG Huan, JIA Yiwen, CHEN Xiaowei, et al. Separation and purification of squalene from rice oil deodorizer distillate[J]. Journal of Henan University of Technology(Natural Science Edition), 2022, 43(6):30-36.
[2] 汪金宇. 米糠油脱臭馏出物的综合利用[D]. 北京: 北京化工大学, 2016. WANG Jinyu. Comprehensive utilization of deodorized distillate from rice bran oil[D]. Beijing: Beijing University of Chemical Technology, 2016.
[3] 甘欢华,刘钟栋,吴凯,等. 大豆油脱臭馏出物酶法甲酯化工艺研究[J]. 中国油脂, 2020, 45(7):78-81, 92. GAN Huanhua, LIU Zhongdong, WU Kai, et al. Enzymatic methyl esterification of soybean oil deodorized distillate[J]. China Oils and Fats, 2020, 45(7):78-81, 92.
[4] 武文华,关国华,蒋一鸣,等. 响应面法优化生物酶法催化脱臭馏出物甲酯化[J] 中国油脂, 2022, 47(11):143-146. WU Wenhua, GUAN Guohua, JIANG Yiming, et al. Optimization of bioenzyme-catalyzed methyl esterification of deodorized distillates by response surface methodology[J]. China Oils and Fats, 2022, 47(11):143-146.
[5] 吴雪辉,蓝梧涛,容欧. 响应面优化油茶籽油脱臭馏出物中维生素E的提取工艺研究[J]. 中国油脂, 2018, 43(5):113-116. WU Xuehui, LAN Wutao, RONG Ou. Optimization of extraction of vitamin E from deodorizer distillate of oil-tea camellia seed oil by response surface methodology[J]. China Oils and Fats, 2018, 43(5):113-116.
[6] 许超群. 大豆油脱臭馏出物中提取天然维生素E的工艺研究[D]. 杭州:浙江工业大学, 2022. Xu Chaoqun. Process study on the extraction of natural vitamin E from deodorized soybean oil distillate[D]. Hangzhou: Zhejiang University of Technology, 2022.
[7] 代志凯,王义永,赵健,等. 棕榈油脱臭馏出物的甲酯化工艺研究[J]. 中国油脂, 2015, 40(9):46-49. DAI Zhikai, WANG Yiyong, ZHAO Jian, et al. Methyl esterification of palm oil deodorizer distillate[J]. China Oils and Fats, 2015, 40(9):46-49.
[8] 金斐. 大豆脱臭馏出物中浓缩天然VE的酯化工艺研究[D]. 杭州: 浙江工业大学, 2017. JIN Fei. Study on esterification technology of concentrated natural VE in soybean deodorizer distillate[D]. Hangzhou: Zhejiang University of Technology, 2017.
[9] CESARINI S, DIAZ P, NIELSEN P M. Exploring a new, soluble lipase for FAMEs production in water-containing systems using crude soybean oil as a feedstock[J]. Process Biochemistry, 2013, 48(3):484-487.
[10] CHANG M Y, CHAN E S, SONG C P. Biodiesel production catalysed by low-cost liquid enzyme Eversa® Transform 2.0: effect of free fatty acid content on lipase methanol tolerance and kinetic model[J]. Fuel, 2021, 283:119266.
[11] BHATT C, NIELSEN P M, RANCKE-MADSEN A, et al. Combining technology with liquid-formulated lipases for in-spec biodiesel production[J]. Biotechnology and Applied Biochemistry, 2022, 69(1):7-19.
[12] 中华人民共和国国家卫生和计划生育委员会.食品安全国家标准食品中酸价的测定: GB 5009.229—2016[S]. 北京:中国标准出版社, 2017. National Health and Family Planning Commission of the Peoples Republic of China. National food safety standard determination of acid value in foods:GB 5009.229—2016[S]. Beijing: Standards Press of China, 2017.
[13] ZENG L P, HE Y J, JIAO L C, et al. Preparation of biodiesel with liquid synergetic lipases from rapeseed oil deodorizer distillate[J]. Applied Biochemistry and Biotechnology, 2017, 183(3):778-791.
[14] MONTEIRO R R C, ARANA-PE(~overN)A S, DA ROCHA T N, et al. Liquid lipase preparations designed for industrial production of biodiesel. Is it really an optimal solution?[J]. Renewable Energy, 2021, 164:1566-1587.
[15] SUN S D, GUO J J, CHEN X W. Biodiesel preparation from Semen Abutili(Abutilon theophrasti Medic.)seed oil using low-cost liquid lipase Eversa® Transform 2.0 as a catalyst[J]. Industrial Crops and Products, 2021, 169:113643.
[16] LOH J M, AMELIA, GOURICH W, et al. Improved biodiesel production from sludge palm oil catalyzed by a low-cost liquid lipase under low-input process conditions[J]. Renewable Energy, 2021, 177:348-358.
[17] ZHOU Y X, LI K Y, SUN S D. Simultaneous esterification and transesterification of waste phoenix seed oil with a high free fatty acid content using a free lipase catalyst to prepare biodiesel[J]. Biomass and Bioenergy, 2021, 144:105930.
[18] GULDHE A, SINGH B, MUTANDA T, et al. Advances in synthesis of biodieselvia enzyme catalysis: novel and sustainable approaches[J]. Renewable and Sustainable Energy Reviews, 2015, 41:1447-1464.
[19] LOTTI M, PLEISS J, VALERO F, et al. Effects of methanol on lipases: molecular, kinetic and process issues in the production of biodiesel[J]. Biotechnology Journal, 2015, 10(1):22-30.
[20] AMELIA, SONG C P, CHANG M Y, et al. Retention of high-value tocols during enzymatic esterification of palm fatty acid distillate using liquid lipase for improving the economics and sustainability of biodiesel production[J]. Industrial Crops and Products, 2023, 194:116271.
[21] ROSSET D V, WANCURA J H C, UGALDE G A, et al. Enzyme-catalyzed production of FAME by hydroesterification of soybean oil using the novel soluble lipase NS 40116[J]. Applied Biochemistry and Biotechnology, 2019, 188(4):914-926.
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