红树林,16S rRNA,系统进化分析,古菌类群," /> 红树林,16S rRNA,系统进化分析,古菌类群,"/> mangrove, 16S rRNA, phylogenetic analysis, archaeal lineages,"/> 福建漳江口红树林沉积物中的古菌类群
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J4 ›› 2009, Vol. 44 ›› Issue (3): 1-6 .doi:

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福建漳江口红树林沉积物中的古菌类群

张熙颖1,2，许静1，肖静1，徐俊1   

  1. 1. 国家海洋局第三海洋研究所海洋生物遗传资源重点实验室，福建 厦门 361005；
    2. 山东大学微生物技术国家重点实验室， 山东 济南 250100
  • 收稿日期:2008-09-18 修回日期:1900-01-01 出版日期:2009-04-24 发布日期:2009-11-26
  • 通讯作者: 徐俊

Archaeal lineages detected in mangrove sediment in the Zhangjiang estuary of Fujian province

ZHANG Xiying 1,2, XU Jing 1, XIAO Jing 1, XU Jun 1   

  1. 1. Key Lab Of Marine Biogenetic Resources, Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, Fujian, China; 2. State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, Shandong, China
  • Received:2008-09-18 Revised:1900-01-01 Online:2009-04-24 Published:2009-11-26
  • Contact: XU Jun

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摘要：

利用环境16S rRNA基因序列分析技术，对福建漳江口红树林沉积物中存在的古菌类群进行检测。首先提取红树林沉积物的总DNA，并进一步构建环境16S rRNA基因克隆文库。随机挑选文库克隆，根据Amplified rDNA restriction analysis(ARDRA)分析分型后进行测序。对所测序列进行系统进化分析。结果显示，文库中的大部分古菌克隆(95%)分别属于广古菌门(Euryarchaeota)中的Marine benthic group D和泉古菌门(Crenarchaeota)的Miscellaneous crenarchaeotic group和Marine group I等3个已描述的古菌类群， 另有小部分克隆(5%)可能代表广古菌门中新的古菌类群。Miscellaneous crenarchaeotic group类群古菌是红树林沉积物中古菌的优势类群。上述古菌类群的检测加深了人们对于红树林古菌群落结构复杂性及其生态学意义的认识。

关键词: 红树林')">红树林, 16S rRNA, 系统进化分析, 古菌类群

Abstract:

Archaeal lineages in mangrove sediment in the Zhangjiang estuary of Fujian province were detected by environmental 16S rRNA gene sequences phylogenetic analysis. Total DNAs were extracted from the mangrove sediment and an archaeal 16S rRNA gene clones library was constructed. The archaeal 16S rRNA gene clones selected by amplified rDNA restriction analysis(ARDRA)were sequenced and phylogenetically analyzed. The results showed that most of archaeal 16S rRNA gene clones （95%） belonged to three described archaeal lineages, which were the marine benthic group D in the Euryarchaeota phylum, the miscellaneous crenarchaeotic group and the marine group I in Crenarchaeota phylum. A few archaeal clones （5%） constituted partially novel lineages in the Euryarchaeota phylum. The miscellaneous crenarchaeotic group(MCG)crenarchaea was the predominant archaeal group in the mangrove sediment. The archaeal lineages revealed in this study enhanced our understanding of the ecological complexity and importance of archaea in mangrove sediment.

Key words: mangrove')">mangrove, 16S rRNA, phylogenetic analysis, archaeal lineages

中图分类号: 

  • Q938
[1] 郭战胜,张军伟,李亮,侯旭光,张海涛,张学进. 3种鲍16S rRNA基因片段分析及系统发育研究[J]. 山东大学学报（理学版）, 2017, 52(1): 1-8.
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