高效液相色谱法检测鸡蛋中的三聚氰胺

J4 ›› 2010, Vol. 45 ›› Issue (5): 19-21.

高效液相色谱法检测鸡蛋中的三聚氰胺

魏晋梅,张咏梅,曹禄兴,罗玉柱*

甘肃农业大学研究测试中心, 甘肃兰州 730070

收稿日期:2009-08-20 出版日期:2010-05-16 发布日期:2010-05-24
通讯作者: 罗玉柱(1962-),男,教授,博士生导师,研究方向为现代生物技.
作者简介:魏晋梅(1973-),女,助理研究员,硕士, 研究方向为食品安全与检测,色谱分析. Email:wkunwjm@126.com
基金资助:
甘肃农业大学创新基金资助项目(000003)

Determination of melamine in eggs by high performance liquid chromatography

WEI Jin-mei, ZHANG Yong-mei, CAO Lu-xing, LUO Yu-zhu*

Instrumental Research and Analysis Center, Gansu Agricultural University, Lanzhou 730070, Gansu, China

Received:2009-08-20 Online:2010-05-16 Published:2010-05-24

摘要/Abstract

摘要：

建立了高效液相色谱法检测鸡蛋中三聚氰胺的方法。样品用1％三氯乙酸和2.2％乙酸铅超声提取。色谱柱为Hypersil ODS 100×4.6mm,5μm;流动相为乙腈:辛烷磺酸钠离子对试剂(pH=3)=15∶85(V／V);流速为1mL／min;检测波长为210nm;柱温为40℃。三聚氰胺在0.2μg／mL~10μg／mL范围内,有良好的线性关系。在空白样品中添加0.2μg／mL、0.4μg／mL、1.0μg／mL三个质量浓度的标准工作液,回收率在82.0%~104.0％,相对标准偏差为6.81％(n=9)。

关键词: 三聚氰胺;鸡蛋;高效液相色谱法

Abstract:

A method was reported for the determination of melamine in eggs. After being extracted by 1％Trichloroacetic acid solution and 2.2％ lead acetate from samples,melamine was separated on a Hypersil ODS 100×4.6mm,5μm C18 column with a volume ratio of acetonitrile and sulfonic sodium octane(pH=3) of 15∶85, and was detected by an ultraviolet detector at a wavelength of 210nm.The temperature of column was 40℃. The linear ranges were from 0.2μg／mL to 10.0μg／mL. The results indicated that the average recoveries were 82.0％~104.0％ for high, middle and low concentration levels by this method. The relative standard deviations RSD was 6.81％(n=9).

Key words: melamine; eggs; high performance liquid chromatography(HPLC)

魏晋梅,张咏梅,曹禄兴,罗玉柱*. 高效液相色谱法检测鸡蛋中的三聚氰胺[J]. J4, 2010, 45(5): 19-21.

WEI Jin-mei, ZHANG Yong-mei, CAO Lu-xing, LUO Yu-zhu*. Determination of melamine in eggs by high performance liquid chromatography[J]. J4, 2010, 45(5): 19-21.

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