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山东大学学报（理学版） ›› 2017, Vol. 52 ›› Issue (1): 1-8.doi: 10.6040/j.issn.1671-9352.0.2016.268

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3种鲍16S rRNA基因片段分析及系统发育研究

郭战胜1,张军伟1,李亮1,侯旭光1*,张海涛1,张学进2   

  1. 1.山东大学(威海)海洋学院, 山东 威海 264209;2.威海市环翠区海洋与渔业研究所, 山东 威海 264200
  • 收稿日期:2016-06-14 出版日期:2017-01-20 发布日期:2017-01-16
  • 通讯作者: 侯旭光(1963— ),男,博士,副教授,研究方向为发育生物学. E-mail:richardhoukk@163.com E-mail:guomarine@163.com
  • 作者简介:郭战胜(1987— ),男,硕士,实验员,研究方向为贝类遗传育种. E-mail:guomarine@163.com
  • 基金资助:
    山东省科技发展计划资助项目(2014GHY115014)

Sequence analysis of mitochondrial 16S rRNA gene and molecular phylogeny of three species of abalones

GUO Zhan-sheng1, ZHANG Jun-wei1, LI Liang1, HOU Xu-guang1*, ZHANG Hai-tao1, ZHANG Xue-jin2   

  1. 1. College of Marine, Shandong University, Weihai 264209, Shandong, China;
    2. Weihai Huancui district of Marine and Fisheries Research Institute, Weihai 264200, Shandong, China
  • Received:2016-06-14 Online:2017-01-20 Published:2017-01-16

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摘要： 运用PCR技术对皱纹盘鲍(Haliotis discus hannai)、黑足鲍(H.iris)和黑唇鲍(H.rubra)3种鲍16S rRNA基因扩增,纯化后测序。结果表明:皱纹盘鲍、黑足鲍和黑唇鲍序列长度分别为544、546、550 bp,序列AT含量分别为58.1%、56.1%和55.9%,三者序列同源性高达94.18%,碱基变异位点分别集中在9~34 bp、248~295 bp、326~375 bp。所得结果与Genebank中22种鲍相应序列进行对比分析,25种鲍遗传距离距离在0.00~0.14之间;用NJ和ML法绘制系统发育树分析其亲缘关系,结果表明鲍科系统发育呈现出一定的区域性,并且染色体数目相同的物种容易聚为一支。

关键词: 皱纹盘鲍, 黑唇鲍, 系统发育, 16S rRNA, 黑足鲍

Abstract: PCR method was used for amplification of mitochondrial 16S rRNA gene from H.discus hannai, H.iris and H.rubra, and all of amplicons were purified and sequenced. The results showed that 16S rRNA gene sequences with length of 544, 546 and 550 bp were obtained from H.discus hannai, H.iris and H.rubra, the content of A+T were 58.1%, 56.1% and 55.9% respectively, the mutual homology among them was 94.18%, the variation of the sequences focuses on the three regions: 9-34 bp, 248-295 bp and 326-375 bp. The cluster dendrograms of the three collected abalones and 22 abalones from GenBank data based on 16s rDNA were analysed, their genetic distance ranges from 0.00 to 0.14. The phylogenetic tree was constructed with NJ and ML methods to reveal the evolutionary relationships, the results showed that the phylogeny of Haliotide was easy to cluster in one clade when distributed in same district and had same chrosome numbers.

Key words: phylogeny, H.rubra, H.iris, H.discus hannai, 16S rRNA

中图分类号: 

  • S917.4
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