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山东大学学报（理学版） ›› 2015, Vol. 50 ›› Issue (11): 1-7.doi: 10.6040/j.issn.1671-9352.0.2014.376

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染色体驱动蛋白KIF4A抑制胃癌细胞的迁移运动

胡晓晴1,3, 梁爽爽1,3, 冀美超1,3, 朱长军1,2,3, 潘丽娜1,2,3   

  1. 1. 天津师范大学生命科学学院, 天津 300387;
    2. 天津市动植物抗性重点实验室, 天津 300387;
    3. 天津师范大学分子细胞系统生物学重点实验室, 天津 300387
  • 收稿日期:2014-08-20 修回日期:2015-01-15 出版日期:2015-11-20 发布日期:2015-12-09
  • 通讯作者: 潘丽娜(1982-),女,博士,讲师,研究方向为驱动蛋白与有丝分裂相关研究.E-mail:skypln@mail.tjnu.edu.cn E-mail:skypln@mail.tjnu.edu.cn
  • 作者简介:胡晓晴(1991-),女,硕士研究生,研究方向为细胞分子生物学.E-mail:feiyuqingyuan1991@126.com
  • 基金资助:
    国家自然科学基金资助项目(31271485);2011年教育部"新世纪优秀人才支持计划"资助项目(NCET-11-1066);天津市应用基础与前沿技术研究计划重点资助项目(12JC2DJC21400);国家自然科学基金青年基金资助项目(31301138);天津师范大学中青年教授学术创新推进计划资助项目(52XB1104);天津师范大学博士基金资助项目(52XC1001);天津师范大学"渤海学者"基金资助项目

Chromokinesin KIF4A inhibits migration of gastric cancer cells

HU Xiao-qing1,3, LIANG Shuang-shuang1,3, JI Mei-chao1,3, ZHU Chang-jun1,2,3, PAN Li-na1,2,3   

  1. 1. College of Life Science, Tianjin Normal University, Tianjin 300387, China;
    2. Tianjin Key Laboratory of Animal and Plant Resistance, Tianjin 300387, China;
    3. Key Laboratory of Molecular and Cellular Systems Biology, Tianjin Normal University, Tianjin 300387, China
  • Received:2014-08-20 Revised:2015-01-15 Online:2015-11-20 Published:2015-12-09

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摘要： 探究染色体驱动蛋白KIF4A与胃癌细胞迁移运动的关系,从而为胃癌临床治疗提供新的治疗靶点。用G418筛选的方法构建了两种细胞株:KIF4A低表达的胃癌SGC细胞株与KIF4A过表达的胃癌SGC细胞株,Western与免疫荧光的验证后,对其分别进行细胞划痕与Transwell迁移实验检测KIF4A对细胞迁移运动能力的影响。成功构建了KIF4A低表达的SGC细胞株(SGC-shKIF4A)与KIF4A过表达的细胞株(SGC-KIF4A),在KIF4A低表达的SGC细胞株中细胞的迁移速率增加,在KIF4A过表达的细胞株中细胞的迁移速率减慢。染色体驱动蛋白KIF4A抑制胃癌细胞的迁移运动能力,为进一步研究KIF4A与胃癌转移的机制奠定基础。

关键词: KIF4A, 迁移, 胃癌, 驱动蛋白

Abstract: To explore the role of chromokinesin KIF4A regulating migration of gastric cancer cells and then provide a potential therapeutic targets for clinical treatment of gastric cancer. Gastric cancer cell lines were constructed with either stably lower-expression or stably over-expression of KIF4A using G418 selection. Then we verified these cell lines using Western Bolt and immunofluorescent staining. These cells' migration abilities were assayed with wound healing and Transwell experiments. KIF4A stably lower-expression (SGC-shKIF4A) or overexpression cell lines (SGC-KIF4A) were successfully constructed and cell migration of SGC-shKIF4A cells were faster than that of SGC-KIF4A cell lines. Chromokinesin KIF4A inhibits the ability of gastric cancer cell movement laying a foundation for studies of KIF4A's roles in gastric cancer metastasis.

Key words: chromokinesin, Gastric cancer, cell migration, KIF4A

中图分类号: 

  • Q78
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