3D打印丝素蛋白-Ⅱ型胶原软骨支架

doi:10.6040/j.issn.1671-9352.0.2017.501

山东大学学报（理学版） ›› 2018, Vol. 53 ›› Issue (3): 82-87.doi: 10.6040/j.issn.1671-9352.0.2017.501

3D打印丝素蛋白-Ⅱ型胶原软骨支架

袁清献^1,2,3,高丽兰^1,2,*,李瑞欣^3*,刘迎节³,林祥龙^1,2,张西正³

1.天津理工大学天津市先进机电系统设计与智能控制重点实验室, 天津 300384;2.天津理工大学机电工程国家级实验教学示范中心, 天津 300384;3.中国人民解放军军事医学科学院卫生装备研究所, 天津 300161

收稿日期:2017-09-27 出版日期:2018-03-20 发布日期:2018-03-13
通讯作者: 高丽兰(1978— ),女,副教授,博士,研究方向为生物力学. E-mail:gaolilan780921@163.com;李瑞欣(1975— ),女,副研究员,博士,研究方向为生物材料. E-mail:limxinxin@163.com E-mail:heyedemeng@163.com
作者简介:袁清献(1989— ),男,硕士研究生,研究方向为生物力学. E-mail:heyedemeng@163.com
基金资助:
国家自然科学基金资助项目(11572222,11432016);天津市自然科学基金资助项目(16JCYBJC28400)

Silk fibroin-type Ⅱ collagen cartilage scaffold fabricated by 3D printing technology

YUAN Qing-xian^1,2,3, GAO Li-lan^1,2*, LI Rui-xin^3*, LIU Ying-jie³, LIN Xiang-long^1,2, ZHANG Xi-zheng³

1. Tianjin Key Laboratory of the Design and Intelligent Contro of the Advanced Mechatronic System, Tianjin University of Technology, Tianjin 300384, China;
2. National Demonstration Center for Experimental Mechanical and Electrical Engineering Education, Tianjin University of Technology, Tianjin 300384, China;
3. Institute of Medical Equipment, Academy of Military Medical Science, Tianjin 300161, China

Received:2017-09-27 Online:2018-03-20 Published:2018-03-13

摘要/Abstract

摘要： 运用三维制图软件Solidworks设计了软骨支架宏观结构,采用3D打印技术和冷冻干燥技术制备了丝素蛋白-Ⅱ型胶原软骨支架。通过实验测试了支架的密度、孔隙率和弹性模量;在支架上接种软骨细胞后,采用MTT法、HE染色和扫描电镜观察3种方法分析了细胞在支架上的增殖和形态。结果显示,丝素蛋白-Ⅱ型胶原软骨支架弹性模量具有率相关性,即随着应变率增加,支架的弹性模量增大;支架的密度和孔隙率分别为(0.086 6±0.008 4)g/cm³和(89.3±3.26)%。支架接种细胞培养7 d后,细胞生长增殖加快;HE染色观察发现,细胞在表层区生长最多,深层区最少;扫描电镜观察发现,支架孔径形状规则,通透性较好,细胞多分布于孔壁表面。

关键词: 丝素蛋白-Ⅱ型胶原, 软骨支架, 率相关, 细胞增殖

Abstract: The macroscopic structure of cartilage scaffold was designed by using Solidworks, and the silk fibroin-type Ⅱ collagen cartilage scaffold was prepared by 3D printing technique and freeze-drying technique. The density, porosity and elastic modulus of the scaffolds were tested by experiments. The proliferation of the cells was analyzed by MTT assay, HE staining and scanning electron microscopy. The results show that the silk fibroin-type II collagen scaffold is dependent on the strain rate. The elastic modulus of scaffold increases with the increase of strain rate. The density and porosity of scaffold were(0.086 6±0.008 4)g/cm³ and(89.3±3.26)%, respectively. The cell growth and proliferation were accelerated after 7 days of inoculation. By analyzing the results of HE staining, it is found that the cells grow most in the surface area and there are the least cells in the deep region. The microscopic images by Scanning electron microscopy(SEM)reveal that the diameter of scaffold is regular and the permeability is better. The cells are mostly distributed on the surface of the spine.

Key words: cartilage scaffold, rate-dependent, cell proliferation, silk fibroin-type Ⅱ collagen

中图分类号:

R318.01

袁清献,高丽兰,李瑞欣,刘迎节,林祥龙,张西正. 3D打印丝素蛋白-Ⅱ型胶原软骨支架[J]. 山东大学学报（理学版）, 2018, 53(3): 82-87.

YUAN Qing-xian, GAO Li-lan, LI Rui-xin, LIU Ying-jie, LIN Xiang-long, ZHANG Xi-zheng. Silk fibroin-type Ⅱ collagen cartilage scaffold fabricated by 3D printing technology[J]. JOURNAL OF SHANDONG UNIVERSITY(NATURAL SCIENCE), 2018, 53(3): 82-87.

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3D打印丝素蛋白-Ⅱ型胶原软骨支架

Silk fibroin-type Ⅱ collagen cartilage scaffold fabricated by 3D printing technology

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