JOURNAL OF SHANDONG UNIVERSITY(NATURAL SCIENCE) ›› 2015, Vol. 50 ›› Issue (01): 42-49.doi: 10.6040/j.issn.1671-9352.0.2014.286

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Genetic diversities of wolves mtDNA D-loop in northwestern Mongolian

LIU Yuan-chao1,2, ZHAO Jing3, CHU Hong-jun1,2,4, DONG Tan-cheng4, HAN Li-li1,2, TAO Yong-shan2, GE Yan2   

  1. 1. College of Resources and Environment Sciences, Xinjiang University, Urumqi 830046, Xinjiang, China;
    2. Altay Management Station, Mt. Kalamaili Ungulate Nature Reserve, Altay 836500, Xinjiang, China;
    3. College of Biology Sciences, Shandong University, Jinan 250100, Shandong, China;
    4. College of Animal Science, Xinjiang Agricultural University, Urumqi 830052, Xinjiang, China
  • Received:2014-06-23 Revised:2015-01-04 Online:2015-01-20 Published:2015-01-24

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Abstract: Genetic diversities of mitochondrial DNA (mtDNA) D-loop and maternal origin of northwestern Mongolian Canis lupus were investigated. Thirty 519 bp mtDNA D-loop sequences were analyzed by PCR and sequencing techniques, and the phylogenetic trees were constructed based on the mtDNA D-loop sequences of northwestern Mongolian Canis lupus samples and other wolves D-loop sequence available from NCBI. There were 7 unique haplotypes with 29 polymorphic sites in northwestern Mongolian Canis lupus, which account for 4.9% of 519 bp sequence analyzed. The haplotype diversity(h) and nucleotide diversity(π) were 0.952±0.096 and 0.011 60±0.006 46, respectively. Moreover, northwestern Mongolian Canis lupus distributed in two branches, and are closely related to Xinjiang of China, Inner Mongolia of China, whereas they are less related to Qinghai of China, Tibet of China, and the Himalayan population. The northwest Mongolia Canis lupus have rather abundant genetic diversity and were greatly affected by other Canis lupus species in history. Qinghai of China, Tibet of China, and the Himalayan Canis lupus should belong to a group.

Key words: Canis lupus, genetic diversity, mtDNA D-loop, maternal origin

CLC Number: 

  • Q38
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