JOURNAL OF SHANDONG UNIVERSITY(NATURAL SCIENCE) ›› 2018, Vol. 53 ›› Issue (3): 82-87.doi: 10.6040/j.issn.1671-9352.0.2017.501

Previous Articles     Next Articles

Silk fibroin-type Ⅱ collagen cartilage scaffold fabricated by 3D printing technology

YUAN Qing-xian1,2,3, GAO Li-lan1,2*, LI Rui-xin3*, LIU Ying-jie3, LIN Xiang-long1,2, ZHANG Xi-zheng3   

  1. 1. Tianjin Key Laboratory of the Design and Intelligent Contro of the Advanced Mechatronic System, Tianjin University of Technology, Tianjin 300384, China;
    2. National Demonstration Center for Experimental Mechanical and Electrical Engineering Education, Tianjin University of Technology, Tianjin 300384, China;
    3. Institute of Medical Equipment, Academy of Military Medical Science, Tianjin 300161, China
  • Received:2017-09-27 Online:2018-03-20 Published:2018-03-13

PDF (PC)

624

Abstract: The macroscopic structure of cartilage scaffold was designed by using Solidworks, and the silk fibroin-type Ⅱ collagen cartilage scaffold was prepared by 3D printing technique and freeze-drying technique. The density, porosity and elastic modulus of the scaffolds were tested by experiments. The proliferation of the cells was analyzed by MTT assay, HE staining and scanning electron microscopy. The results show that the silk fibroin-type II collagen scaffold is dependent on the strain rate. The elastic modulus of scaffold increases with the increase of strain rate. The density and porosity of scaffold were(0.086 6±0.008 4)g/cm3 and(89.3±3.26)%, respectively. The cell growth and proliferation were accelerated after 7 days of inoculation. By analyzing the results of HE staining, it is found that the cells grow most in the surface area and there are the least cells in the deep region. The microscopic images by Scanning electron microscopy(SEM)reveal that the diameter of scaffold is regular and the permeability is better. The cells are mostly distributed on the surface of the spine.

Key words: cartilage scaffold, rate-dependent, cell proliferation, silk fibroin-type Ⅱ collagen

CLC Number: 

  • R318.01
[1] LIU M, LIU N, ZANG R, et al. Engineering stem cell niches in bioreactors[J]. World Journal of Stem Cells, 2013, 5(4):124-135.
[2] BOSE S, ROY M, BANDYOPADHYAY A. Recent advances in bone tissue engineering scaffolds[J]. Trends in Biotechnology, 2012, 30(10):546-554.
[3] ZHANG X, REAGAN M R, KAPLAN D L. Electrospun silk biomaterial scaffolds for regenerative medicine[J]. Advanced Drug Delivery Reviews, 2009, 61(12):988-1006.
[4] YIN L H, PENG P, MU X, et al. Preparation and characterization of three dimensional porous silk fibroin/gelatin composite scaffolds[J]. Journal of Functional Materials, 2013, 44(23):3388-3391.
[5] FENG X X, ZHANG L L, CHEN J Y, et al. Preparation and characterization of novel nanocomposite films formed from silk fibroin and nano-TiO2.[J]. International Journal of Biological Macromolecules, 2007, 40(2):105-111.
[6] KWANSA A L, DE V R, FREEMAN J W. Tensile mechanical properties of collagen type I and its enzymatic crosslinks[J]. Biophysical Chemistry, 2016, s 214/215:1-10.
[7] 秦胜男. Ⅱ型胶原—透明质酸复合支架材料的构建及在软骨组织工程应用的初步研究[D]. 广州:暨南大学, 2010. QIN Shengnan. The construction of collagen type Ⅱ-hyaluronic acid composite biomaterial and preliminary application in the tissue-engineering cartilage[D]. Guangzhou: Jinan University, 2010.
[8] CATROS S, GUILLEMOT F, NANDAKUMAR A, et al. Layer-by-layer tissue microfabrication supports cell proliferation in vitro and in vivo.[J]. Tissue Engineering Part C Methods, 2012, 18(18):62-70.
[9] 周惠琼, 吴东海, 李东民. 应用酶解及氯化钠盐析方法对4个种属Ⅱ型胶原的提纯及比较[J]. 中华医学杂志, 2001, 81(11):696-697. ZHOU Huiqiong, WU Donghai, LI Dongmin, et al. Purification and comparison of four species type II collagen by enzymolysis and sodium chloride salting out[J]. Chinese Medical Journal, 2001, 81(11):696-697.
[10] KAPFER S C, HYDE S T, MECKE K, et al. Minimal surface scaffold designs for tissue engineering[J]. Biomaterials, 2011, 32(29):6875-6882.
[11] YOO D J. Computer-aided porous scaffold design for tissue engineering using triply periodic minimal surfaces[J]. International Journal of Precision Engineering & Manufacturing, 2011, 12(1):61-71.
[12] SUN W, LAL P. Recent development on computer aided tissue engineering-a review[J]. Computer Methods & Programs in Biomedicine, 2002, 67(2):85-103.
[13] GAO L L, ZHANG C Q, DONG L M, et al. Description of depth-dependent nonlinear viscoelastic behavior for articular cartilage in unconfined compression[J]. Materials Science & Engineering C, 2012, 32(2):119-125.
[14] OTTANI V, RASPANTI M, RUGGERI A. Collagen structure and functional implications[J]. Micron, 2001, 32(3):251-260.
[15] DIAO H J, FUNG H S, YEUNG P, et al. Dynamic cyclic compression modulates the chondrogenic phenotype in human chondrocytes from late stage osteoarthritis[J]. Biochemical & Biophysical Research Communications, 2017, 486(1):14-21.
[16] ZHOU F, ZHANG X, CAI D, et al. Silk fibroin-chondroitin sulfate scaffold with immuno-inhibition property for articular cartilage repair[J]. Acta Biomaterialia, 2017, 63(2):64-75.
[17] 陈隆坤. 双层胶原/大孔径PLA纳米纤维支架用于关节骨软骨组织工程的研究[D].杭州:浙江大学,2011. CHEN Longkun. Fabrication of bilaver collagen/microporous nanofiber scaffolds and its application to articular osteochondral tissue engineering[D]. Hangzhou: Zhejiang University, 2011.
[18] CASTRO-CESEÑA A B, CAMACHO-VILLEGAS T A, LUGO-FABRES P H, et al. Effect of starch on the mechanical and in vitro properties of collagen-hydroxyapatite sponges for applications in dentistry[J]. Carbohydr Polym, 2016, 148(1):78-85.
[19] ZHU H, WU B, FENG X, et al. Preparation and characterization of bioactive mesoporous calcium silicate—silk fibroin composite films[J]. Sichuan Journal of Physiological Sciences, 2011, 98(2):330-341.
[20] ZHU H, JIAN J, SHEN J. Biomacromolecules electrostatic self-assembly on 3-dimensional tissue engineering scaffold.[J]. Biomacromolecules, 2004, 5(5):1933-1939.
[21] WHANG K, GOLDSTICK T K, HEALY K E. A biodegradable polymer scaffold for delivery of osteotropic factors[J]. Biomaterials, 2000, 21(24):2545-2551.
[1] LUAN Yi-chao, YANG Xiu-ping, ZHANG Jing-jing, LIU Qing, ZHANG Chun-qiu. Finite element simulation of relaxation properties on lumbarintervertebal disc under compression [J]. JOURNAL OF SHANDONG UNIVERSITY(NATURAL SCIENCE), 2018, 53(3): 77-81.
[2] YANG Xiu-ping, LUAN Yi-chao, ZHANG Jing-jing, LIU Qing, ZHANG Chun-qiu. Creep experiments study on lumbar intervertebral disc under different loading conditions [J]. JOURNAL OF SHANDONG UNIVERSITY(NATURAL SCIENCE), 2017, 52(5): 31-36.
[3] FU Hu, CHEN Ling, MEN Yu-tao, JIANG Yan-long. Experiment and finite element analysis of articular cartilage under rolling load [J]. JOURNAL OF SHANDONG UNIVERSITY(NATURAL SCIENCE), 2017, 52(5): 37-40.
[4] ZHANG Jing-jing,YANG Xiu-ping,LIU Qing,ZHANG Chun-qiu*. Mechanics response analysis of lumbar intervertebral disc based on Biot theory [J]. JOURNAL OF SHANDONG UNIVERSITY(NATURAL SCIENCE), 2016, 51(11): 93-98.
[5] CHEN Ling, MEN Yu-tao, WANG Jia-jiang. Reliability analysis of dental implantation and split-root technique joint repaired postoperative dental [J]. JOURNAL OF SHANDONG UNIVERSITY(NATURAL SCIENCE), 2016, 51(5): 6-10.
[6] WANG Jia-jiang, CHEN Ling, MEN Yu-tao, JI Chen. The feasibility study on shorten treatment cycle of dental implantation and split-root technique joint repair [J]. JOURNAL OF SHANDONG UNIVERSITY(NATURAL SCIENCE), 2016, 51(3): 40-43.
[7] WANG Long-tao, YANG Xiu-ping, LIU Qing, YANG Wen-jing, FAN Zhen-min, ZHANG Chun-qiu. Solute transport in articular cartilage under rolling-compressing load [J]. JOURNAL OF SHANDONG UNIVERSITY(NATURAL SCIENCE), 2015, 50(01): 81-84.
[8] JIANG Jun, YANG Xiu-ping, LIU Qing, ZHANG Chun-qiu. Simulating the solute diffusion in articular cartilage under compression loading [J]. JOURNAL OF SHANDONG UNIVERSITY(NATURAL SCIENCE), 2015, 50(01): 85-89.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Copyright 2018 © Editorial office of JOURNAL OF SHANDONG UNIVERSITY(NATURAL SCIENCE)
Supported by Beijing Magtech Co.Ltd