JOURNAL OF SHANDONG UNIVERSITY(NATURAL SCIENCE) ›› 2025, Vol. 60 ›› Issue (9): 31-40.doi: 10.6040/j.issn.1671-9352.0.2025.031

Previous Articles    

Mechanism of Resina Draconis in promoting healing of diabetic foot wounds

YANG Ziyu, DAI Zhaoyuan, LI Gen*, FENG Xinchi   

  1. School of Chinese Materia Medica, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China
  • Published:2025-09-10

PDF (PC)

2

Abstract: Network pharmacology, molecular docking, and experimental verification were employed to investigate the mechanism by which Resina Draconis promotes the healing of diabetic foot wounds. The TCMSP database was utilized to screen the chemical constituents and targets of Resina Draconis. Disease targets were retrieved from the GeneCards and DisGeNET databases using keywords such as “diabetic foot ulcer” and “diabetic wound”. A “component-disease-target” network diagram was created using the intersections of component targets and disease targets. Bioinformatic analysis predicted the mechanism of Resina Draconis in facilitating wound healing. Molecular docking was used to investigate the combination of the components of Resina Draconis and key targets. Mouse macrophages were used to study the effects of Resina Draconis and its main components on the expression of the receptor of advanced glycation end products(RAGE)protein. The results showed that 226 common disease targets were obtained by network pharmacological screening. KEGG enrichment analysis suggested that the effect of Resina Draconis on diabetic ulcers may be associated with the AGE-RAGE pathway. The results of molecular docking demonstrated that loureirin A, loureirin B, loureirin C, loureirin D, resveratrol, and 7,4'-dihydroxyflavone exhibited good binding activity with the key target RAGE. Resina Draconis and its main components could significantly reverse the abnormal expression of RAGE protein in mouse macrophages induced by advanced glycation end products(AGEs). In conclusion, the mechanism of Resina Draconis in promoting healing of diabetic foot ulcers was associated with the regulation of the AGE-RAGE pathway and the inhibition of the abnormal expression of the RAGE protein caused by the accumulation of advanced glycation end products(AGEs).

Key words: Resina Draconis, diabetic foot, network pharmacology, molecular docking, RAGE

CLC Number: 

  • R285
[1] ZHOU M G, WANG H D, ZENG X Y, et al. Mortality, morbidity, and risk factors in China and its provinces, 1990—2017: a systematic analysis for the Global Burden of Disease Study 2017[J]. The Lancet, 2019, 394(10204):1145-1158.
[2] ARMSTRONG D G, TAN T W, BOULTON A J M, et al. Diabetic foot ulcers: a review[J]. JAMA, 2023, 330(1):62-75.
[3] FU X B. Wound healing center establishment and new technology application in improving the wound healing quality in China[J]. Burns & Trauma, 2020, 8:38.
[4] HICKS C W, SELVARAJAH S, MATHIOUDAKIS N, et al. Burden of infected diabetic foot ulcers on hospital admissions and costs[J]. Annals of Vascular Surgery, 2016, 33:149-158.
[5] ARMSTRONG D G, BOULTON A J M, BUS S A. Diabetic foot ulcers and their recurrence[J]. New England Journal of Medicine, 2017, 376(24):2367-2375.
[6] SORG H, TILKORN D J, HAGER S, et al. Skin wound healing: an update on the current knowledge and concepts[J]. European Surgical Research Europaische Chirurgische Forschung Recherches Chirurgicales Europeennes, 2017, 58(1/2):81-94.
[7] BURGESS J L, WYANT W A, ABDO ABUJAMRA B, et al. Diabetic wound-healing science[J]. Medicina, 2021, 57(10):1072.
[8] CHANG M, NGUYEN T T. Strategy for treatment of infected diabetic foot ulcers[J]. Accounts of Chemical Research, 2021, 54(5):1080-1093.
[9] 杨敏,杨瑞,覃彬华,等. 民族药龙血竭研究进展[J]. 海峡药学,2019,31(11):49-52. YANG Min, YANG Rui, QIN Binhua, et al. Research progress of dragons blood, a national medicine[J]. Strait Pharmaceutical Journal, 2019, 31(11):49-52.
[10] 孙晶,庞道然,郑姣,等. 龙血竭的药理、临床及质量控制研究进展[J]. 中国医院用药评价与分析,2017,17(11):1445-1447. SUN Jing, PANG Daoran, ZHENG Jiao, et al. Research progress in pharmacology, clinic and quality control of Resina Draconis[J]. Evaluation and Analysis of Drug-Use in Hospitals of China, 2017, 17(11):1445-1447.
[11] FAN J Y, YI T, SZE-TO C M, et al. A systematic review of the botanical, phytochemical and pharmacological profile of Dracaena cochinchinensis, a plant source of the ethnomedicine “dragons blood”[J]. Molecules, 2014, 19(7):10650-10669.
[12] 牟蕾,李秀萍. 龙血竭胶囊治疗糖尿病足溃疡病人的临床研究[J]. 双足与保健,2019,28(13):3-4. MU Lei, LI Xiuping. Clinical study of dragon blood dragon capsule in the treatment of diabetic foot ulcer patients[J]. Biped and Health, 2019, 28(13):3-4.
[13] 孟庆元,林炜栋,陈向芳. AGE-RAGE系统与糖尿病足综合征的发病机制及治疗进展[J]. 药学服务与研究,2009,9(2):118-121. MENG Qingyuan, LIN Weidong, CHEN Xiangfang. The relationship between AGE-RAGE system and pathogenesis of diabetic foot syndrome, and advance on treatment[J]. Pharmaceutical Care and Research, 2009, 9(2):118-121.
[14] BOEZIO B, AUDOUZE K, DUCROT P, et al. Network-based approaches in pharmacology[J]. Molecular Informatics, 2017, 36(10):28692140.
[15] 廖韵诺,赵凯丽,郭宏伟. 中药网络药理学的研究应用与挑战[J]. 中草药,2024,55(12):4204-4213. LIAO Yunnuo, ZHAO Kaili, GUO Hongwei. Application and challenges of network pharmacology research in traditional Chinese medicine[J]. Chinese Traditional and Herbal Drugs, 2024, 55(12):4204-4213.
[16] 林炜栋,陆树良,青春. 糖尿病难愈创面与晚期糖基化终末产物的关系[J]. 中国临床康复,2003,7(17):2491-2493. LIN Weidong, LU Shuliang, QING Chun. The relation between impaired wound healing and advanced glycation end products[J]. Modern Rehabilitation, 2003, 7(17):2491-2493.
[17] LITWINOFF E, HURTADO DEL POZO C, RAMASAMY R, et al. Emerging targets for therapeutic development in diabetes and its complications: the RAGE signaling pathway[J]. Clinical Pharmacology and Therapeutics, 2015, 98(2):135-144.
[18] TAKAHASHI H K, MORI S J, WAKE H, et al. Advanced glycation end products subspecies-selectively induce adhesion molecule expression and cytokine production in human peripheral blood mononuclear cells[J]. The Journal of Pharmacology and Experimental Therapeutics, 2009, 330(1):89-98.
[19] WANG Q, ZHU G Y, CAO X Z, et al. Blocking AGE-RAGE signaling improved functional disorders of macrophages in diabetic wound[J]. Journal of Diabetes Research, 2017, 2017:1428537.
[20] TWARDA-CLAPA A, OLCZAK A, BIAŁKOWSKA A M, et al. Advanced glycation end-products(AGEs): formation, chemistry, classification, receptors, and diseases related to AGEs[J]. Cells, 2022, 11(8):1312.
[21] WU J T, TU M C, ZHUNG P. Advanced glycation end product(AGE): characterization of the products from the reaction between D-glucose and serum albumin[J]. Journal of Clinical Laboratory Analysis, 1996, 10(1):21-34.
[1] NING Shuyi, DAI Zhaoyuan, FENG Xinchi. Chemical composition analysis and the determination of main components of Resina Draconis based on UPLC-QE-Orbitrap-MS [J]. JOURNAL OF SHANDONG UNIVERSITY(NATURAL SCIENCE), 2025, 60(9): 10-23.
[2] WU Xianxi, HUANG Danjun. Equitable coloring of graphs with mad(G)≤(13)/4 [J]. JOURNAL OF SHANDONG UNIVERSITY(NATURAL SCIENCE), 2025, 60(2): 41-50.
[3] Liang CHEN, Chengwei FANG, Ji MENG, Xiuping MA, Dan ZHU, Jiaxin LI. Mechanism of Acotus tatarinowii Schott in treating Alzheimer's disease [J]. JOURNAL OF SHANDONG UNIVERSITY(NATURAL SCIENCE), 2024, 59(11): 64-73, 92.
[4] Yiran LI,Ning ZHAO,Zhijian ZHANG. Prediction of average queue time in multi-server tandem queueing systems [J]. JOURNAL OF SHANDONG UNIVERSITY(NATURAL SCIENCE), 2024, 59(1): 17-26.
[5] SU Xiao-yan, CHEN Jing-rong, YIN Hui-ling. Generalized interval-valued Pythagorean triangular fuzzy aggregation operator and application in decision making [J]. JOURNAL OF SHANDONG UNIVERSITY(NATURAL SCIENCE), 2022, 57(8): 77-87.
[6] LI Rui-feng, YI Zheng-ge, YUAN Wen-yong, YANG Xiao-yuan, NIU Ke. ECDSA-based auditing scheme for cloud storage [J]. JOURNAL OF SHANDONG UNIVERSITY(NATURAL SCIENCE), 2022, 57(5): 57-65.
[7] HUANG Lin, YUAN Xiu-jiu, SUO Zhong-ying, PANG Meng-yang, BAO Zhuang-zhuang, LI Zhi-wei. Interval-valued q-rung hesitant fuzzy frank aggregation operators and their application in multi-attribute decision making [J]. JOURNAL OF SHANDONG UNIVERSITY(NATURAL SCIENCE), 2021, 56(3): 54-66.
[8] DENG Xiao-qin, WU Qun-ying. Precise asymptotics of complete moment convergence for ρ-mixing sequence [J]. JOURNAL OF SHANDONG UNIVERSITY(NATURAL SCIENCE), 2020, 55(6): 32-40.
[9] MA Hai-feng, YANG Jia-hai, XUE Qing-shui, JU Xing-zhong, ZHU Hao-zhi, LIN Tao, YUAN Xin-xin. A not-same frequency remote data possession checking method [J]. JOURNAL OF SHANDONG UNIVERSITY(NATURAL SCIENCE), 2020, 55(5): 81-87.
[10] WANG Min, FANG Xiao-zhen, QU Meng, SHU Li-sheng. Boundedness for commutator of multilinear Hardy-Littlewood maximal operator [J]. JOURNAL OF SHANDONG UNIVERSITY(NATURAL SCIENCE), 2020, 55(2): 16-22.
[11] YAO Ke, YANG Hai-ning, QIN Jing. Searchable encryption with data integrity verification in cloud storage [J]. JOURNAL OF SHANDONG UNIVERSITY(NATURAL SCIENCE), 2019, 54(1): 67-78.
[12] LIU Li-zhao, YU Jia-ping, LIU Jian, LI Jun-yi, HAN Shao-bing, XU Hua-rong, LIN Huai-chuan, ZHU Shun-zhi. Secure storage addressing algorithm for large data based on quantum radiation field [J]. JOURNAL OF SHANDONG UNIVERSITY(NATURAL SCIENCE), 2018, 53(7): 65-74.
[13] ZHANG Jiang-yue, XU Chang-qing. Linear 2-arboricity of graphs with maximum average degree at most 4 [J]. JOURNAL OF SHANDONG UNIVERSITY(NATURAL SCIENCE), 2018, 53(6): 7-10.
[14] PAN Wen-hua, XU Chang-qing. Neighbor sum distinguishing index of a kind of sparse graphs [J]. JOURNAL OF SHANDONG UNIVERSITY(NATURAL SCIENCE), 2017, 52(8): 94-99.
[15] JU Rui, WANG Li-na, YU Rong-wei, XU Lai. A method of storing heterogeneous monitoring data for cloud platform [J]. JOURNAL OF SHANDONG UNIVERSITY(NATURAL SCIENCE), 2017, 52(5): 104-110.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Copyright 2018 © Editorial office of JOURNAL OF SHANDONG UNIVERSITY(NATURAL SCIENCE)
Supported by Beijing Magtech Co.Ltd