J4 ›› 2011, Vol. 46 ›› Issue (11): 1-4.

• Articles •     Next Articles

 Agrobacterium-mediated introduction of disease-resistant genes
into tomato(Lycopersicon esculentum Mill

YU Hui-min1, SHI Zhu1, SHAO Hong-wei1, LI Wei1, HOU Bing-kai2   

  1. 1. Department of Biology, Qilu Normal University, Jinan 250013, Shandong, China; 
    2. Key Laboratory of Plant Cell Engineering and Germplasm Innovation, Ministry of Education,
    Shandong University, Jinan 250100, Shandong, China
  • Received:2010-12-20 Online:2011-11-20 Published:2011-11-30


A plant expression vector pBLGC containing two disease-resistant genes, the Chitinase gene and the Glutanase gene, was introduced into cotyledon explants of tomato via Agrobacterium-mediated genetic transformation. After co-culture, kanamycin selection and regeneration, 23 kanamycin-resistant plants were obtained. Among these, 7 plants were demonstrated to be transgenics by PCR and PCR-Southern Blot analysis. Through pathogenic fungus inoculation, it was shown that 3 transgenic plants had strong disease resistance. These results will lay an important basis for the further study of tomato disease resistance and for tomato genetic improvement.

Key words: Agrobacterium tumefaciens; tomato; chitinase; glucanase; disease resistance; genetic transformation

No related articles found!
Full text



No Suggested Reading articles found!