J4 ›› 2011, Vol. 46 ›› Issue (7): 11-16.
• Articles •
ZHANG Bi-cheng1, HU Lin2*, LIU Yan-hui2, ZHANG Jing1
The interaction, at different time, different temperature and different concentration, between FLRX、LMF and Salmon sperm DNA are studied by UV-visible spectrophotometry. The results showed that Salmon sperm DNA′s UV adsorption values increas with the concentration of both quinolones increasing respectively. Two quinolones bind DNA′s groove at pH4.0～11.0. The combination constants of between FLRX、LMF and Salmon sperm DNA are calculated by double reciprocal analysis,and the combination constants are KDNAFLRX=0223×104L/mol and KDNALMF=0.290×104L/mol.
Salmon sperm DNA; UV spectroscopy; FLRX; LMF
ZHANG Bi-cheng1, HU Lin2*, LIU Yan-hui2, ZHANG Jing1. Study on interaction of both quinolones with DNA by ultraviolet spectroscopy[J].J4, 2011, 46(7): 11-16.
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