J4 ›› 2011, Vol. 46 ›› Issue (7): 11-16.

• Articles • Previous Articles     Next Articles

Study on interaction of both quinolones with DNA by ultraviolet spectroscopy

ZHANG Bi-cheng1, HU Lin2*, LIU Yan-hui2, ZHANG Jing1   

  1. 1. College of Life Sciences, Guizhou University, Guiyang 550025, Guizhou, China;
    2. College of Sciences, Guizhou University, Guiyang 550025, Guizhou, China
  • Received:2010-12-14 Online:2011-07-20 Published:2011-09-08

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Abstract:

The interaction, at different time, different temperature and different concentration, between FLRX、LMF and Salmon sperm DNA are studied by UV-visible spectrophotometry. The results showed that Salmon sperm DNA′s UV adsorption values increas with the concentration of both quinolones increasing respectively. Two quinolones bind DNA′s groove at pH4.0～11.0. The combination constants of between FLRX、LMF and Salmon sperm DNA are calculated by double reciprocal analysis,and the combination constants are KDNAFLRX=0223×104L/mol and KDNALMF=0.290×104L/mol.

Key words:  Salmon sperm DNA; UV spectroscopy; FLRX; LMF

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