J4 ›› 2011, Vol. 46 ›› Issue (7): 21-25.
• Articles •
JIN Zhi, HU Nai-feng, LIU Qiang, WANG Pu*
The synthetic Tbfmoc-Met-OH is used to replace Fmoc-Met-OH as a building block for solid phase peptide synthesis of ubiquitin. Based on the significant differences in gel filtration and UV absorbance between the Tbfmocubiquitin and the impurities, Tbfmocubiquitin can be separated from the impurities using gel filtration easily and efficiently. After removing the Tbfmoc group under mild condition (pH=8.5), the ubiquitin product is obtained with high purity, which is confirmed by amino acid analysis, MS and HPLC measurements. The strategy used in the paper can overcome the Tbfmoc-Cl’s weak points, including long reaction time, low coupling efficiency, more by-product producing and product purification difficulty, and the established new method can be used for the synthesis, separation and purification of ubiquitin easily and efficiently.
Tbfmoc-Met-OH; ubiquitin; solid phase peptide synthesis
JIN Zhi, HU Nai-feng, LIU Qiang, WANG Pu*. Application of TbfmocMetOH for solid phase synthesis of ubiquitin[J].J4, 2011, 46(7): 21-25.
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