关键词: 烟叶微生物, 水解酶, 人工陈化 , 温度稳定性, 诱导产生

Abstract: The inducible production and temperature stability of five hydrolases (βamylase, glucoamylase, cellulases, pectinase and protease) of microorganisms from fluecured tobacco leaves were studied. The results indicate that the production of five enzymes could be induced effectively by adding 0.2% inducements to 2.0% tobacco leaf water media. Five enzymes are unstable incubated at 60℃, while four enzymes had preferable temperature stability at 50℃ except for pectinase. All the enzymes have higher enzyme activities reacted at 60℃ than that reacted at the temperature stipulated by enzyme activity assays. Using microorganisms and their hydrolases, six experimentations were carried out with two kinds of fluecured tobacco leaves for a sixmonth artificial aging. Three batch samples were taken for analyses of internal quality during the period. The results of the assessment of sense organ with smoking show the total scores of the first batch samples are increased. This research provide the foundation for shortening the time of aging and improving the internal quality of fluecured tobacco leaves.

Key words: artificial aging , temperature stability, inducible production, hydrolases, tobacco microorganisms