墨鱼黑色素纳米球pH响应机制及其对毛鳞片光保护的影响

doi:10.6040/j.issn.1671-9352.0.2025.109

摘要/Abstract

摘要： 本研究针对墨鱼黑色素在化妆品应用中存在的结构异质性及难溶性难题,创新性地提出pH调控介导的结构重塑策略,系统揭示黑色素分散特性与功能演变之间的构效关系。通过多尺度结构表征(透射电子显微镜(TEM)/扫描电子显微镜(SEM))发现,碱性处理可诱导黑色素发生显著结构重组,其天然致密球形颗粒(中性pH)转化为具有高表面活性的纤维-无定形复合结构,该重构显著提升其分散度和界面结合能力。在此基础上,以人发纤维为模型构建光损伤防护体系,通过FT-IR光谱证实经碱处理的黑色素可通过氢键网络与角蛋白形成稳定复合物,紫外-可见光谱进一步显示其对UVA/UVB波段吸收效率提升。尤其值得关注的是,共聚焦显微分析表明改性黑色素能在毛鳞片间隙形成连续光防护膜,较天然黑色素的离散分布模式,其紫外线反射率降低。该研究为开发基于海洋生物质的高效光防护化妆品提供理论依据与技术路径。

关键词: 墨鱼黑色素, pH敏感, 光防护

Abstract: The inherent structural heterogeneity and pronounced insolubility of natural cuttlefish melanin, a biomacromolecule, pose critical bottlenecks in material characterization and functional regulation, severely limiting its advanced applications in cosmetics. To overcome these technical limitations, this study innovatively employed a pH-modulated structural remodeling strategy to systematically elucidate the structure-function relationships governing its solubility evolution and functional enhancement. Multi-scale structural analyzed(SEM/TEM)reveal that alkaline treatment induced a phase transition from native compact spherical particles(neutral pH)to fibrous-amorphous composited with elevated surface activity. This structural reconfiguration enhanced aqueous solubility and interfacial binding capacity compared to native melanin. Using human hair fibers as a photodamage protection model, FT-IR spectroscopy confirmed stable keratin-melanin complex formation through reconstructed hydrogen-bond networks following alkaline treatment. UV-Vis spectroscopy demonstrated an improvement in UVA/UVB absorption efficiency(280-400 nm wavelength range). Notably, confocal microscopy revealed that modified melanin forms continuous photoprotective films within cuticle interstices, achieved lower UV reflectivity compared to the discrete distribution pattern of native melanin. This research established a dual theoretical-technical framework for developing marine biomass-derived advanced photoprotective cosmeceuticals, offering insights into biomacromolecule functionalization through supramolecular restructuring.

Key words: cuttlefish melanin, pH sensitivity, light protection

中图分类号:

O648

朱元宇,赵洪德,赵芷晴,王新昊,王继乾,王栋. 墨鱼黑色素纳米球pH响应机制及其对毛鳞片光保护的影响[J]. 《山东大学学报(理学版)》, 2025, 60(10): 127-140.

ZHU Yuanyu, ZHAO Hongde, ZHAO Zhiqing, WANG Xinhao, WANG Jiqian, WANG Dong. pH-responsive mechanism of cuttlefish melanin nanospheres and their effect on photoprotection of hair scales[J]. JOURNAL OF SHANDONG UNIVERSITY(NATURAL SCIENCE), 2025, 60(10): 127-140.

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